Volume 4, Issue 4 (Int J Mol Cell Med 2015)                   Int J Mol Cell Med 2015, 4(4): 235-239 | Back to browse issues page

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Mahdavi poor B, Asgharzadeh M, Fallah E, Hatam-Nahavandi K, Rashedi J, Dalimi A. Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran. Int J Mol Cell Med 2015; 4 (4) :235-239
URL: http://ijmcmed.org/article-1-382-en.html
Molecular Characterization of Cryptosporidium Species in Children with Diarrhea in North West of Iran
Behroz Mahdavi poor1 , Mohammad Asgharzadeh2 , Esmaeel Fallah3 , Kareem Hatam-Nahavandi4 , Jalil Rashedi5 , Abdolhossein Dalimi6
1- Department of Laboratory Science, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran. , Behroz.mahdavi@gmail.com
2- Biotechnology Research Center and Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
3- Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
5- Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
6- Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract:   (9133 Views)

Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tabriz Pediatric Hospital. The amplification of small subunit ribosomal RNA gene was performed using a nested polymerase chain reaction protocol and its products were digested using two restriction enzymes for Cryptosporidium species and genotype differentiation. Cryptosporidium oocysts were found in 2 (1.76%) children with diarrhea and restriction pattern revealed the presence of C.parvum bovine genotype in both positive fecal samples. The findings indicate that Cryptosporidium parvum is responsible for cryptosporidiosis in children in the study region and probably zoonotic transmission is the predominant route of parasite transmission.

Keywords: Child, Cryptosporidium, genotype, PCR, Iran
Full-Text [PDF 58 kb]   (3772 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2015/09/12 | Accepted: 2015/10/30 | Published: 2015/11/29
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