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:: Volume 12, Issue 1 (Int J Mol Cell Med 2023) ::
Int J Mol Cell Med 2023, 12(1): 30-39 Back to browse issues page
Amyloid Beta Alters the Expression of microRNAs Regulating HMGCR and ABCA1 Genes in Astrocytes of C57BL/6J Mice
Hossein Azizi Dariuni1 , Mehrnaz Karimi Darabi1 , Zahra Nazeri1 , Shirin Azizidoost2 , Alireza Kheirollah1 , Azam Khedri1 , Maryam Cheraghzadeh 3
1- Department of Biochemistry, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- Atherosclerosis Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Biochemistry, School of Medicine, Cellular & Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , cheraghzade_m@ajums.ac.ir
Abstract:   (663 Views)
Dysregulation of brain cholesterol homeostasis causes the accumulation of extracellular protein deposits called amyloid plaques in the hippocampus which eventually leads to neuronal death, memory and learning deficits. The aim of the present study was to investigate the effect of beta amyloid on miRNAs regulating HMGCR and ABCA1 as cholesterol synthesis and homeostasis genes. Primary astrocytes were isolated from C57BL/6J mice, and were treated with 0.5 μM amyloid beta (Aβ). Expression levels of genes and miRNAs were measured by real-time PCR. In comparison to control, Aβ treatment resulted in a significant decrease in miR-96-5p expression as a positive and negative regulator of HMGCR and ABCA1, respectively. There was no significant increase in miR-27a-3p expression as a negative regulator of HMGCR. miR-106b-5p and miR-143-3p expressions were also dramatically decreased as ABCA1 negative regulators. Amyloid beta can alter the expression of major genes in the cholesterol homeostasis pathway via their regulatory miRNAs.
Keywords: ABCA1, HMGCR, microRNA, astrocytes, amyloid beta
Full-Text [PDF 1262 kb]   (461 Downloads)    
Type of Study: Original Article | Subject: Clinical Biochemistry
Received: 2023/04/19 | Accepted: 2023/09/17 | Published: 2023/09/19
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Azizi Dariuni H, Karimi Darabi M, Nazeri Z, Azizidoost S, Kheirollah A, Khedri A et al . Amyloid Beta Alters the Expression of microRNAs Regulating HMGCR and ABCA1 Genes in Astrocytes of C57BL/6J Mice. Int J Mol Cell Med 2023; 12 (1) :30-39
URL: http://ijmcmed.org/article-1-2130-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 1 (Int J Mol Cell Med 2023) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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