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Amyloid Beta Alters the Expression of microRNAs Regulating HMGCR and ABCA1 Genes in Astrocytes of C57BL/6J Mice
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Hossein Azizi Dariuni1    , Mehrnaz Karimi Darabi1 , Zahra Nazeri1 , Shirin Azizidoost2 , Alireza Kheirollah1 , Azam Khedri1 , Maryam Cheraghzadeh 3 |
1- Department of Biochemistry, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2- Atherosclerosis Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Biochemistry, School of Medicine, Cellular & Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. , cheraghzade_m@ajums.ac.ir |
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Abstract: (717 Views) |
| Dysregulation of brain cholesterol homeostasis causes the accumulation of extracellular protein deposits called amyloid plaques in the hippocampus which eventually leads to neuronal death, memory and learning deficits. The aim of the present study was to investigate the effect of beta amyloid on miRNAs regulating HMGCR and ABCA1 as cholesterol synthesis and homeostasis genes. Primary astrocytes were isolated from C57BL/6J mice, and were treated with 0.5 μM amyloid beta (Aβ). Expression levels of genes and miRNAs were measured by real-time PCR. In comparison to control, Aβ treatment resulted in a significant decrease in miR-96-5p expression as a positive and negative regulator of HMGCR and ABCA1, respectively. There was no significant increase in miR-27a-3p expression as a negative regulator of HMGCR. miR-106b-5p and miR-143-3p expressions were also dramatically decreased as ABCA1 negative regulators. Amyloid beta can alter the expression of major genes in the cholesterol homeostasis pathway via their regulatory miRNAs. |
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| Keywords: ABCA1, HMGCR, microRNA, astrocytes, amyloid beta |
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Full-Text [PDF 1262 kb]
(519 Downloads)
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Type of Study: Original Article |
Subject:
Clinical Biochemistry
Received: 2023/04/19 | Accepted: 2023/09/17 | Published: 2023/09/19
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