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The Assessment of Early Glycosaminoglycan Concentration Changes in the Kidney of Diabetic Rats by Critical Electrolyte Concentration Staining
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Mohsen Pourghasem1   , Ebrahim Nasiri2 , Shima Sum3 , Hamid Shafi 4 |
1- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
2- Department of anatomical Sciences, GeilanUniversity of Medical Sciences, Rasht, Iran.
3- Department of Social Medicin, Babol University of Medical Sciences, Babol, Iran.
4- Department of Urology, Babol University of Medical Sciences, Babol, Iran. , dr_hamidshafi@yahoo.com |
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Abstract: (11813 Views) |
| Glycosaminoglycan (GAG) has a pivot role in renal function and homeostasis. Analysis of GAG amount generally serves to determine GAG alteration due to diabetes mellitus. Critical Electrolyte Concentration (CEC) staining can be an efficacy method to study GAG amount changes. Based on an experimental study, 20 male rats were randomly divided equally into two experimental and control groups. Diabetes mellitus was induced by a single sub cutaneous injection (120 mg/kg) of alloxan monohydrate. After 8 weeks, diabetic kidneys were paraffin embedded and sectioned at 5μm on a microtome. Slides were prepared and studied after staining by Critical Electrolyte Concentration (CEC 1 -4). In this study, we succeeded to show a decrease of hyaluronic acid and chondroitin sulfate concentration in diabetic kidney at 8 weeks diabetic rats which are earlier signs compared to those reported previously. In contrary, no significant changes in heparin sulfate and keratin sulfate have been seen. Diabetic nephropathy is a progressive disease and earlier diagnosis makes a better treatment design to reduce its development. CEC staining is able to determine degradation of hyaluronic acid and chondroitin sulfate synthesis in diabetic kidney of rats in an earlier time. |
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| Keywords: Glycosaminoglycan, Diabetes mellitus, kidney, CEC |
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Full-Text [PDF 123 kb]
(3244 Downloads)
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Type of Study: Original Article |
Subject:
Clinical Biochemistry
Received: 2013/05/21 | Accepted: 2013/07/13 | Published: 2013/07/13
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