Volume 13, Issue 3 (Int J Mol Cell Med 2024)                   Int J Mol Cell Med 2024, 13(3): 259-271 | Back to browse issues page

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Abbasi Y, Pooladi M, Nazmabadi R, Amri J, Abbasi H, Aghabeygi R et al . Enhancement of the Sensitivity of the Acute Lymphoblastic Leukemia Cells to ABT-737 by Formononetin. Int J Mol Cell Med 2024; 13 (3) :259-271
URL: http://ijmcmed.org/article-1-2341-en.html
Enhancement of the Sensitivity of the Acute Lymphoblastic Leukemia Cells to ABT-737 by Formononetin
Yusef Abbasi1 , Marziyeh Pooladi2 , Roya Nazmabadi3 , Jamal Amri4 , Helia Abbasi5 , Razieh Aghabeygi6 , Hadi Karami 7
1- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran. & Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
2- Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran.
3- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
4- Students’ Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Biology, Faculty of Sciences, Payame Noor University, Hamedan Branch, Hamedan, Iran
6- Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran
7- Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran & Department of Molecular Medicine and Biotechnology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran. , hadimolmed@gmail.com
Abstract:   (267 Views)
Overexpression of (myeloid leukemia cell differentiation protein 1) Mcl-1 is associated with the reduction of ABT-737 toxicity and secondary resistance. In this study, the effect of formononetin (biochanin B) on Mcl-1 expression, cell growth, apoptosis and ABT-737 sensitivity of the acute lymphoblastic leukemia (ALL) cells was investigated. In this experimental study, the cell proliferation and MTT assays were used to investigate the effect of formononetin on cell growth and survival. qRT-PCR was performed for the measurement of gene expression. Hoechst 33342 staining and caspase-3 activity assay were used for the determination of apoptosis. Our data showed that formononetin and ABT-737 both led to a significant reduction in the IC50 value and synergistically reduced the cell growth and survival relative to single treatment. Overexpression of Mcl-1 was found after the treatment with ABT-737. Formononetin decreased the expression of B-cell lymphoma 2 (Bcl-2) and Mcl-1 and increased the Bcl-2-associated protein x (Bax) and P21 expression. Moreover, formononetin enhanced the apoptotic effect of ABT-737 in ALL cells. In summary, formononetin showed anti-carcinogenic activities in human ALL cells via suppression of cell growth and survival. Formononetin enhanced the apoptotic effect of ABT-737, with contribution by inhibition of the Mcl-1 expression.
 
Keywords: ABT-737, ALL, apoptosis, Formononetin, Mcl-1
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Type of Study: Original Article | Subject: Cell Biology
Received: 2024/05/10 | Accepted: 2024/08/14 | Published: 2024/09/11
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