International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
Sat, Apr 26, 2025
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Volume 11, Issue 3 (Int J Mol Cell Med 2022)
Int J Mol Cell Med 2022, 11(3): 236-243 |
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ATEŞ B, ÖNER Ç, AKBULUT Z, ÇOLAK E. Capsaicin Alters the Expression of Genetic and Epigenetic Molecules In Hepatocellular Carcinoma Cell. Int J Mol Cell Med 2022; 11 (3) :236-243
URL: http://ijmcmed.org/article-1-2017-en.html
URL: http://ijmcmed.org/article-1-2017-en.html
Capsaicin Alters the Expression of Genetic and Epigenetic Molecules In Hepatocellular Carcinoma Cell
1- Maltepe University, School of Medicine, İstanbul, Turkey.
2- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey. ,cagri.oner@maltepe.edu.tr
3- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey.
4- Eskişehir Osmangazi University, Medical Faculty, Department of Biostatistics, Eskişehir, Turkey.
2- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey. ,
3- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey.
4- Eskişehir Osmangazi University, Medical Faculty, Department of Biostatistics, Eskişehir, Turkey.
Abstract: (1901 Views)
Capsaicin is a natural product which is extracted from pepper and has the potential to be used in cancer treatment because of its anti- proliferative effects. The aim of the study was to determine the effect of capsaicin on the hepatocellular carcinoma cell proliferation and the expressions of related genetic markers as Ki-67, PI3K/AKT/mTOR and epigenetic markers as miR-126 and piR-Hep-1. The inhibitory concentration of capsaicin in HepG2 cells was determined. piR-Hep-1 and miR-126 expressions and Ki-67, PI3K, AKT and mTOR gene expressions were examined by RT-PCR. The inhibitory concentration of capsaicin for HepG2 cells was 200 nM and the decreased proliferation was observed at 24th hour. As epigenetic markers, an up regulation of miR-126 and down regulation of piR-Hep-1 expression were determined after treatment. Moreover, Ki-67, PI3K and mTOR gene expressions decreased while AKT gene expression increased after the treatment (p<0.001). According to the obtained data, capsaicin has an impact on proliferation both genetically and epigenetically. Furthermore, treatment of capsaicin effects miR-126 and piR-Hep-1 expressions which effect carcinogenesis in different way. Moreover, there are some clues which indicate that these two small non-coding RNA might affect each other and share the same target molecules post-transcriptionally.
Type of Study: Original Article |
Subject:
Cell Biology
Received: 2022/10/22 | Accepted: 2023/05/23 | Published: 2023/06/17
Received: 2022/10/22 | Accepted: 2023/05/23 | Published: 2023/06/17
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