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Capsaicin Alters the Expression of Genetic and Epigenetic Molecules In Hepatocellular Carcinoma Cell
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Beren ATEŞ1    , Çağrı ÖNER 2, Zeynep AKBULUT3 , Ertuğrul ÇOLAK4 |
1- Maltepe University, School of Medicine, İstanbul, Turkey.
2- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey. , cagri.oner@maltepe.edu.tr
3- Maltepe University, Medical Faculty, Department of Medical Biology and Genetics, İstanbul, Turkey.
4- Eskişehir Osmangazi University, Medical Faculty, Department of Biostatistics, Eskişehir, Turkey. |
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Abstract: (1400 Views) |
Capsaicin is a natural product which is extracted from pepper and has the potential to be used in cancer treatment because of its anti- proliferative effects. The aim of the study was to determine the effect of capsaicin on the hepatocellular carcinoma cell proliferation and the expressions of related genetic markers as Ki-67, PI3K/AKT/mTOR and epigenetic markers as miR-126 and piR-Hep-1. The inhibitory concentration of capsaicin in HepG2 cells was determined. piR-Hep-1 and miR-126 expressions and Ki-67, PI3K, AKT and mTOR gene expressions were examined by RT-PCR. The inhibitory concentration of capsaicin for HepG2 cells was 200 nM and the decreased proliferation was observed at 24th hour. As epigenetic markers, an up regulation of miR-126 and down regulation of piR-Hep-1 expression were determined after treatment. Moreover, Ki-67, PI3K and mTOR gene expressions decreased while AKT gene expression increased after the treatment (p<0.001). According to the obtained data, capsaicin has an impact on proliferation both genetically and epigenetically. Furthermore, treatment of capsaicin effects miR-126 and piR-Hep-1 expressions which effect carcinogenesis in different way. Moreover, there are some clues which indicate that these two small non-coding RNA might affect each other and share the same target molecules post-transcriptionally. |
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| Keywords: Capsaicin, miR-126, piR-Hep-1, PI3K/AKT/mTOR signalling |
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Full-Text [PDF 599 kb]
(920 Downloads)
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Type of Study: Original Article |
Subject:
Cell Biology
Received: 2022/10/22 | Accepted: 2023/05/23 | Published: 2023/06/17
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