[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Issue 1 (Int J Mol Cell Med (In Press) 2022) ::
Int J Mol Cell Med 2022, 11(1): 0-0 Back to browse issues page
Sesamin Acts as Anti-leukemic Compound Interacted with Novel Phosphoprotein Targets and Induced Apoptosis in Leukemic Cells
Pattharin Wannapruk1 , Kamolchanok Deesrisak1 , Sittiruk Roytrakul2 , Dalina Tanyong 3
1- Department of clinical microscopy, Faculty of Medical Technology, Mahidol University, Thailand.
2- National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand.
3- Department of clinical microscopy, Faculty of Medical Technology, Mahidol University, Thailand. , dalina.itc@mahidol.ac.th
Abstract:   (182 Views)
Leukemia is one of the high-incidence cancers that characterized by an abnormal production of immature white blood cells. Subjected to many reports on side effects of conventional chemotherapy, herbs and natural compounds have been studied as an alternative medicine. This study, sesamin, a lignan in sesame seed that has pharmaceutical functions including anti-cancer, was chosen and treated with MOLT-4 and NB4 leukemic cell lines in various concentrations for 24 and 48 hours. The effect of sesamin on cell inhibition and expression levels of apoptotic genes in leukemic cell lines were investigated by MTT assay and real-time PCR, respectively. Moreover, apoptotic proteins were studied by mass spectrometry and bioinformatics tools to investigate the relation between sesamin and targeted proteins. Results showed that sesamin increased cell inhibition in both cell lines in dose- and time-dependent manner. Levels of caspase-3, -7, -8, and -9 gene expressions were significantly increased, while BCL-2 was decreased drastically in sesamin-treated cells. From bioinformatics study, PARP4, IPPK and caspase family proteins were found to be involved in sesamin induced apoptosis in leukemic cells. Besides, doxorubicin, a chemotherapeutic drug, also shared the same protein targets as sesamin in apoptosis pathway. Sesamin demonstrates its potential to enhance cell inhibition and promote cell apoptosis in both MOLT-4 and NB4 leukemic cell lines. This study would be benefit for the development of sesamin as an effective anti-leukemia drug in the future.
 
Keywords: Sesamin, leukemia, MOLT-4, NB4, apoptosis, Doxorubicin
Full-Text [PDF 569 kb]   (52 Downloads)    
Type of Study: Original Article | Subject: Cancer
Received: 2022/03/8 | Accepted: 2022/09/7 | Published: 2022/06/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Wannapruk P, Deesrisak K, Roytrakul S, Tanyong D. Sesamin Acts as Anti-leukemic Compound Interacted with Novel Phosphoprotein Targets and Induced Apoptosis in Leukemic Cells. Int J Mol Cell Med. 2022; 11 (1)
URL: http://ijmcmed.org/article-1-1886-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 1 (Int J Mol Cell Med (In Press) 2022) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4460