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Long Non-coding RNA snaR Promotes Proliferation in EGFR Wild Type Non-Small Cell Lung Cancer Cells
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Melika Ameli Mojard1    , Mandana Ameli Mojarad1 , Alireza Pourmahdian 2 |
1- Department of Biology, Faculty of Basic Sciences, Kharrazi University, Tehran, Iran.
2- Department of Molecular Medicine, Tehran University of Medical Sciences, Tehran, Iran. , dr.rezaamahdian@gmail.com |
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Abstract: (3414 Views) |
| Lung cancer is the second most common cancer and has high morbidity and mortality worldwide with non-small cell lung cancer (NSCLC) accounting for 85% of the cases. Over-expression of epidermal growth factor receptor (EGFR) has been clarified in different cancers, and has been shown to have a crucial role in tumor progression. In this study, we evaluated long non-coding RNA small NF90-associated RNA (snaR) expression in different EGFR-statue cell lines. Knockdown experiments were conducted to analyze snaR expression in selected cell lines. MTT and transwell assays were respectively employed to evaluate the proliferative and invasive abilities of NSCLC cells. The expression of snaR was remarkably up-regulated in SPC-A1 and A549 wild-type EGFR cell lines. Down regulation of snaR with small interfering RNA significantly inhibited cell invasion as well as proliferation of SPC-A1 and A549 cells. Our results indicate that snaR may be a potential therapeutic biomarker for NSCLC. |
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| Keywords: Long non-coding RNA, snaR, non-smallcell lung carcinoma, EGFR |
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Full-Text [PDF 342 kb]
(953 Downloads)
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Type of Study: Original Article |
Subject:
Cancer
Received: 2021/06/7 | Accepted: 2022/01/4 | Published: 2022/06/6
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