International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
Sun, Dec 22, 2024
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Volume 10, Issue 3 (Int J Mol Cell Med 2021)
Int J Mol Cell Med 2021, 10(3): 207-217 |
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Rezaie F, Mokhtari M J, Kalani M. Quercetin Arrests in G2 phase, Upregulates INXS LncRNA and Downregulates UCA1 LncRNA in MCF-7 Cells. Int J Mol Cell Med 2021; 10 (3) :207-217
URL: http://ijmcmed.org/article-1-1540-en.html
URL: http://ijmcmed.org/article-1-1540-en.html
1- Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
2- Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran. ,Mj.mokhtari@gmail.com
3- Department of Immunology, Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Biology, Zarghan Branch, Islamic Azad University, Zarghan, Iran. ,
3- Department of Immunology, Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract: (3532 Views)
One of the most prevalent malignancies, which have severe effects on women's health, is breast cancer. Quercetin, a flavonoid found in vegetables, tea, and fruits, is known to have bioactive properties, such as anti-inflammatory, anti-oxidant, as well as anti-cancer. Long non-coding RNAs (lncRNAs) have been recognized to function as primary regulators of diverse cellular processes, including differentiation, development, and cell fate. INXS and UCA1 are lncRNAs that are upregulated and downregulated, respectively in cancer cells. This research aimed to assess the impact of quercetin on the expression of INXS and UCA1 genes in MCF-7 cells. Various quercetin concentrations at different times were used to treat MCF-7 cells. The cell viability and IC50 values were determined using MTT assay. Then, MCF-7 cells were incubated with various quercetin concentrations for 24, 48, and 72 h. Cell cycle analyzes were evaluated by flow cytometry. The levels of INXS and UCA1 genes expression compared with the GAPDH gene at different concentrations of quercetin were quantified using real-time PCR method. Based on the results, quercetin exerted a dose- and time-dependent inhibitory impact on the viability of MCF-7 cells. Furthermore, quercetin induced cell cycle arrest at the G2 phase in MCF-7 cells. Also, quercetin induced INXS upregulation and UCA1 downregulation in the MCF-7 cell line. These data suggest that quercetin might increase cell death by upregulating INXS and downregulating UCA1 lncRNAs in MCF-7 cells.
Type of Study: Original Article |
Subject:
Cancer
Received: 2021/01/1 | Accepted: 2021/10/30 | Published: 2022/01/10
Received: 2021/01/1 | Accepted: 2021/10/30 | Published: 2022/01/10
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