A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines

Ehsani, Maryam; Zabihi, Ebrahim; Gharouee, Hamed

Volume 1, Issue 4 (Int J Mol Cell Med 2012) Int J Mol Cell Med 2012, 1(4): 197-202 | Back to browse issues page

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Ehsani M, Zabihi E, Gharouee H. A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines. Int J Mol Cell Med 2012; 1 (4) :197-202
URL: http://ijmcmed.org/article-1-63-en.html

A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines

Maryam Ehsani¹

, Ebrahim Zabihi²

, Hamed Gharouee³

1- Dental Materials Research Center, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.
2- Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. , e.zabihi@mubabolac.ir
3- Department of Endodontics, School of Dentistry, Babol University of Medical Sciences, Babol, Iran.

Abstract: (15191 Views)

The aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2Seal and AH Plus, in two osteoblast-like cell lines, MG-63 and Saos-2. Using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. The extracts were exchanged with Saos-2 or MG-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability tests were performed for each extract and cell line using MTT and trypan blue dye exclusion assays. Corresponding incubated media were used as negative control groups. For both extracts and sealers, cytotoxicity was observed in both cell lines. For Saos-2, there was no statistical difference in toxicity between the sealers for either extract (p > 0.05). For MG-63, the 2Seal 24-h extract and the AH Plus 72-h extract had greater cytotoxicity than the other extracts (p < 0.05(. Both AH Plus and 2Seal demonstrated significant cytotoxicity in these two cell lines. In contrast to 2Seal, the cytotoxicity of AH Plus in the MG-63 cell line increased with extraction time from 24 to 72 h. The AH Plus and 2Seal 24-h extracts showed different levels of cytotoxicity in the MG-63 cell line, while in the Saos-2 cell line there were no detectable differences. This may reflect higher sensitivity of the MG-63 cell line compared to Saos-2 toward cytotoxicity induced by these two sealers, or different kinetics of toxicant release from the sealers.

Keywords: Cytotoxicity, AH Plus, 2Seal, Osteosarcoma, Saos-2, MG-63

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Type of Study: Original Article | Subject: Cell Biology
Received: 2013/02/2 | Accepted: 2013/04/9 | Published: 2013/04/9