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Stigmasterol- an Acetylcholinesterase Inhibitor from Phormidium retzii with relevance to Alzheimer’s disease Therapy
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Rincy Yesudas    1, Vinoth Kumar Thirumalairaj2 , Geetharamani Durairaj3 , Amrutha Chacko2 , Lakshmanasenthil Shanmugaasokan4 , Suja Gunasekaran2 |
1- CMS College of Science and Commerce, Chinnavedampatti (PO), Coimbatore, Tamil Nadu, India. , drrincyyesudas@gmail.com
2- CMS College of Science and Commerce, Chinnavedampatti (PO), Coimbatore, Tamil Nadu, India.
3- Dr. N.G.P. Arts and Science College, Coimbatore, Tamil Nadu, India.
4- CAS in Marine Biology, Annamalai University, Chidambaram, Tamil Nadu, India. |
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Abstract: (904 Views) |
| This study observed in vitro screening, purification and identification of cholinesterase inhibitors from the microalgae Phormidium retzii. Mixed microalgal culture was screened from freshwater samples for Phormidium sp. Single colony was purified and authenticated as P. retzii. Acetylcholinesterase (AChE) enzyme was purified from hRBC ghost. Sequential extraction of P. retzii was performed using organic solvents. Cholinesterase enzyme activity and its inhibition by various extracts were then tested. The active fractions were then subjected to partial purification and characterization. Petroleum ether extract of P. retzii showed maximum inhibition of 68.6 % against AChE while other solvent extracts showed no inhibition. Seven fractions were obtained from the active extract using thin layer chromatography. Among which fraction no. 5 showed maximum inhibition of 86.37 % towards AChE. Fraction no. 5 when subjected to GC-MS led to determination of the active principle as stigmasterol. The maximum inhibition of stigmasterol (0.45µM) was 81.2±0.08% with IC50 value of 0.214. Stigmasterol from P. retzii inhibited AChE projecting itself as safer drug for Alzheimer’s disease with minimal side effects. |
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| Keywords: Acetylcholinesterase, Alzheimer’s disease, inhibition, microalgae, Phormidium retzii, stigmasterol |
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Full-Text [PDF 433 kb]
(421 Downloads)
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Type of Study: Original Article |
Subject:
Other
Received: 2023/02/8 | Accepted: 2023/11/12 | Published: 2023/12/16
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