International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
Thu, Nov 21, 2024
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Volume 12, Issue 3 (Int J Mol Cell Med 2023)
Int J Mol Cell Med 2023, 12(3): 220-228 |
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Secer Celik F, Eroglu Gunes C, Kurar E. Cardiac Glycoside Oleandrin Suppresses EMT Ability in Endometrial Carcinoma Cells. Int J Mol Cell Med 2023; 12 (3) :220-228
URL: http://ijmcmed.org/article-1-2118-en.html
URL: http://ijmcmed.org/article-1-2118-en.html
1- Department of Medical Biology and Genetics, Faculty of Medicine, Ankara Medipol University, Ankara, Turkey. , bio.secer@gmail.com
2- Department of Medical Biology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
2- Department of Medical Biology, Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
Abstract: (715 Views)
Endometrial carcinoma is one of the most common types of cancer among women. The progression of cancer occurs via the Epithelial- Mesenchymal Transition (EMT) pathway. Cells lose their epithelial properties and become mobile. For this reason, the EMT process is one of the most important step to be targeted in cancer treatment. Oleandrin is a cardiac glycoside and its use is limited due to its narrow therapeutic index. In this study, we aimed to evaluate effects of lower level Oleandrin doses on EMT process in endometrial carcinoma. Oleandrin was administrated to Ishikawa endometrial adenocarcinoma cells at different doses and times. IC50 dose was determined by XTT proliferation test. Expression analysis of EMT-related genes was then performed by qRT-PCR. Invasion and colony formation abilities of cells were examined microscopically. Finally, the migration analysis of cancer cells was determined by the Wound Healing Assay. The IC50 dose of Oleandrin applied to Ishikawa cells was determined as 75.3 nM at the 48 h. According to qRT-PCR analysis, expression levels of ZEB1, FN1, ITGB1, VIM, SMAD2, SNAI1, SNAI2, SNAI3, and TGFB3 genes significantly decreased, but TIMP2, TIMP3, ITGAV and GSK3B genes significantly increased. In addition, Oleandrin significantly reduced colony formation and invasion of Ishikawa cells. According to the Wound Healing analysis, the migratory abilities of the Oleandrin-treated cells were reduced compared to the control. Low dose Oleandrin suppresses the EMT pathway in Ishikawa cells. It has been shown that Oleandrin significantly suppresses the cell's colony formation, invasion and migration ability both in gene expression analyzes and microscopically.
Type of Study: Original Article |
Subject:
Cancer
Received: 2023/02/26 | Accepted: 2024/04/6 | Published: 2024/04/30
Received: 2023/02/26 | Accepted: 2024/04/6 | Published: 2024/04/30
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