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Applying Vasopressin-Pre-Conditioned Human Adipose Mesenchymal Stem Cells Improves Heart Condition after Transplantation into Infarcted Myocardium
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Shakiba Nasiri Boroujeni1    , Farzaneh Chehelcheraghi2 , Mojtaba Khaksarian1 , Mehrnoosh Sedighi3 , Vajihe Ghorbanzadeh2 , Afshin Nazari 4 |
1- Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran.
2- Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
3- Cardiovascular Research Center, Shahid Rahimi Hospital, Lorestan University of Medical Sciences, Khoramabad, Iran.
4- Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran. , nazary257@yahoo.com |
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Abstract: (1452 Views) |
| Transplantation of H-AdMSCs may improve heart function after MI. AVP is a neurohypophyseal hormone that reduces cardiovascular damage. This study investigated the role of AVP preconditioning in the survival of MSCs and their effect on myocardial repair in the MI rats. H-AMSCs were isolated and incubated for 3 days. The expression of oxytocin and vasopressin receptors was evaluated by Real-time-PCR. Forty male Wistar rats were divided into 4 groups: control, sham, ASC and AVP-ASC. Ischemia was established by ligation of LAD coronary artery. Electrocardiography, fibrosis, angiogenesis, and apoptosis in myocardium were determined after 7 days. Results showed that preconditioned MSCs significantly increased cardiac function when compared with group that received non-preconditioned MSCs. This was associated with significantly reduced fibrosis, increased vascular density, and decreased resident myocyte apoptosis. Results indicate that AVP preconditioned MSCs can be consider a novel approach to management of MI. |
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| Keywords: Myocardial infarction, Arginine vasopressin, fibrosis, apoptosis, angiogenesis, mesenchymal stem cells |
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Full-Text [PDF 719 kb]
(672 Downloads)
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Type of Study: Original Article |
Subject:
Stem Cell
Received: 2022/08/25 | Accepted: 2023/04/24 | Published: 2023/05/2
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