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:: Volume 1, Issue 3 (Int J Mol Cell Med 2012) ::
Int J Mol Cell Med 2012, 1(3): 153-161 Back to browse issues page
Molecular analysis of 18S rRNA gene of Cryptosporidium parasites from patients living in Iran, Malawi, Nigeria and Vietnam
Salman Ghaffari1, Narges Kalantari 2
1- Parasitology and Mycology Group, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
2- Cellular and Molecular Biology Research Centre, Babol University of Medical Sciences, Babol, Iran. , nfkala@gmail.com
Abstract:   (9184 Views)

  Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S rRNA gene in Cryptosporidium for exact parasite identification .

  DNA was extracted from 26 fecal samples from which initially Cryptosporidium oocysts were identified by Ziehl-Neelsen acid-fast , Auramine phenol and ELISA techniques. Nested PCR, targeting the most polymorphic region of the 18S rRNA gene and genotyping was performed by restriction endonuclease digestion of the PCR product followed by nucleotide sequencing and phylogenic analysis.

  Among 26 isolates analyzed, three species of Cryptosporidium were identified 38.5% of the isolates were C. hominis while 53.8% of the isolates were C. parvum and 7.7% of the isolates were C. meleagridis, which the last two species have the potentially zoonotic transmission. The only 11T subtype of C. hominis was demonstrated. These strains clustered distinctly into either human or animal origin regardless of the geographical origin, age, or immunity status of the patients.

In summary, this work is the first report of C. meleagridis infecting human in Iran. Moreover, it suggested that multi-locus study of Cryptosporidium species in developing countries would be necessary to determine the extent of transmission of cryptosporidiosis in the populations.
Keywords: 18S rRNA, Cryptosporidium, diarrhea, developing countries
Full-Text [PDF 281 kb]   (1947 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2013/01/12 | Accepted: 2013/03/11 | Published: 2013/03/11
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Ghaffari S, Kalantari N. Molecular analysis of 18S rRNA gene of Cryptosporidium parasites from patients living in Iran, Malawi, Nigeria and Vietnam. Int J Mol Cell Med. 2012; 1 (3) :153-161
URL: http://ijmcmed.org/article-1-57-en.html


Volume 1, Issue 3 (Int J Mol Cell Med 2012) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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