[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 5, Number 3 (Int J Mol Cell Med 2016) ::
Int J Mol Cell Med 2016, 5(3): 167-177 Back to browse issues page
Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by cAMP-elevating Agents
Atefeh Shahbazi1, Fatemeh Alikarami2, Saeid Kargozar3, Mohammad Hossein Asadi4, Mohammad Taghi Joghataei5, Mansoureh Soleimani5, Majid Safa *6
1- Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran., Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
2- Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran., Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran., Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4- Department of Anatomy, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran., Department of Anatomy, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
5- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran., Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
6- Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran., Department of Hematology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. , safa.m@iums.ac.ir
Abstract:   (2245 Views)

Human umbilical cord matrix (hUCM) is considered as a promising source of mesenchymal stem cells (MSCs) due to several advantages over other tissues. The potential of neural differentiation of hUCM-MSCs is of great interest in the context of treating neurodegenerative diseases. In recent years, considerable efforts have been made to establish in vitro conditions for improving the differentiation of hUCM-MSCs toward neuronal cells. In the present study, we evaluated the neural differentiation potential of hUCM-MSCs in the presence of cAMP-elevating agents forskolin and 3-isobutyl-1-methylxanthine (IBMX). hUCM-MSCs were isolated from fetal umbilical cord and characterized by flow cytometry analysis for mesenchymal specific markers. Mesodermal differentiation potential was assessed through selective media with lineage-specific induction factors. For assessment of neural differentiation, cells were cultured in the presence of cAMP-elevating agents for 8 and 24 h. The neuronal differentiated MSCs were characterized for neuronal specific markers by immunocytochemistry and western blotting. Isolated hUCM-MSCs were found positive for mesenchymal markers (CD73, CD90, and CD105) while negative for hematopoietic markers (CD34 and CD45) .Following neural induction, most cells represented neural-like cells morphology. Neural markers including β-tubulin III (Tuj-1), neuron-specific enolase (NSE), microtubule-associated protein-2 (MAP-2) and nestin were expressed in treated cells with respect to control group. The astrocyte specific marker, glial fibrillary acidic protein (GFAP) was also shown by immunofluorescence in treated cells. (These findings demonstrate that hUCM-MSCs have the ability to rapidly differentiate into neural cell types of neuron-like cells and astrocytes by cAMP-elevating agents without the presence of growth factors.

Keywords: Umbilical cord matrix mesenchymal stem cells, neural differentiation, cAMP
Full-Text [PDF 283 kb]   (861 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2016/06/17 | Accepted: 2016/08/14 | Published: 2016/09/13
Send email to the article author

Add your comments about this article
Your username or email:

Write the security code in the box >



DOI: 10.22088/acadpub.BUMS.5.3.167


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shahbazi A, Alikarami F, Kargozar S, Asadi M H, Joghataei M T, Soleimani M et al . Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by cAMP-elevating Agents. Int J Mol Cell Med. 2016; 5 (3) :167-177
URL: http://ijmcmed.org/article-1-540-en.html
Volume 5, Number 3 (Int J Mol Cell Med 2016) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.046 seconds with 805 queries by yektaweb 3506