Achillea biebersteinii-Mediated Gold Nanoparticles on Oocyte Gene Expression and Follicular Development in Mice

Abdollahi, Mohammadreza; Zangooei, Mohammad; Ghorbani, Parishad; Mahmoudian, Reihaneh Alsadat; Mansouri, Atena

doi:10.22088/IJMCM.BUMS.14.4.1072

Volume 14, Issue 4 (Int J Mol Cell Med 2025) Int J Mol Cell Med 2025, 14(4): 1072-1083 | Back to browse issues page

‎ 10.22088/IJMCM.BUMS.14.4.1072

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Abdollahi M, Zangooei M, Ghorbani P, Mahmoudian R A, Mansouri A. Achillea biebersteinii-Mediated Gold Nanoparticles on Oocyte Gene Expression and Follicular Development in Mice. Int J Mol Cell Med 2025; 14 (4) :1072-1083
URL: http://ijmcmed.org/article-1-2577-en.html

Achillea biebersteinii-Mediated Gold Nanoparticles on Oocyte Gene Expression and Follicular Development in Mice

Mohammadreza Abdollahi¹

, Mohammad Zangooei²

, Parishad Ghorbani³

, Reihaneh Alsadat Mahmoudian⁴

, Atena Mansouri⁵

1- Department of Anatomy, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran & Innovated Medical Research Center & Department of Immunology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
2- Department of Nursing, Qaen School of Medical Sciences, Geriatric Health Research Center, Birjand University of Medical Sciences, Birjand, Iran.
3- Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
4- Cancer research Center, Mashhad University of Medical Sciences, Mashhad, Iran & Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
5- Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran , mansouri_atena@yahoo.com

Abstract: (729 Views)

This research aimed to compare the effects of chemical gold nanoparticles (GNPs) and green gold nanoparticles (gGNPs) derived from Achillea bielineeinii (AB) extract (gGNP) on antrum diameter. The expression of genes linked to follicular development in NMRI mice. Real-time PCR and ImageJ software were utilized to evaluate gene expression and antrum diameter, respectively.

The gGNPs notably increased the antrum diameter of secondary follicles at a dose of 100 μg/ml compared to that of the GNPs at the same dosage (p ≤ 0.05). The antrum diameter of the transplant follicles did not significantly differ across the experimental groups or from the control group. The real-time PCR results showed an increase in the expression of all studied genes (BMP-15, GDF-9, SOD, and GPx) in response to both GNP and gGNPs. However, the impact of gGNPs on gene expression was significantly more than that of GNPs (p ≤ 0.05), except for for the SOD gene. Overall, these findings suggest that the use of AB enhances the effects of GNP on parameters associated with follicular development.

Keywords: Gold Nanoparticles, Achillea biebersteinii, Expression, Gene, Antrum

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Systematic Reviews and Meta-Analyses: Original Article | Subject: Cell Biology
Received: 2025/04/12 | Accepted: 2025/10/1 | Published: 2025/07/28