International Journal of Molecular and Cellular Medicine (IJMCM)
Babol University of Medical Sciences
Tue, Nov 4, 2025
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Volume 14, Issue 3 (Int J Mol Cell Med 2025)
Int J Mol Cell Med 2025, 14(3): 872-885 |
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Khoshkholgh Pahlaviani M R M, Emami A, Mahmoodi S M M, Rezaeian A A. Evaluation of properties of Lactobacillus delbrueckii as an antitumor agent: effects in cell cycle, apoptosis, and gene expression in HT-29 colorectal cancer cells. Int J Mol Cell Med 2025; 14 (3) :872-885
URL: http://ijmcmed.org/article-1-2558-en.html
URL: http://ijmcmed.org/article-1-2558-en.html
Mohammad Reza Majid Khoshkholgh Pahlaviani1 
, Amir Emami2 , Seyed Mohammad Mehdi Mahmoodi3 , Abbas Ali Rezaeian4
, Amir Emami2 , Seyed Mohammad Mehdi Mahmoodi3 , Abbas Ali Rezaeian4
1- Department of Microbiology, La.C, Islamic Azad University, Lahijan, Iran.
2- Burn & Wound Healing Research Center, Shi.C, Shiraz, Iran.
3- Department of Microbiology, Kaz.C, Islamic Azad University, Kazerun, Iran.
4- Department of Microbiology, Ja.C, Islamic Azad University, Jahrom, Iran. ,AA.Rezaeian@iau.ac.ir
2- Burn & Wound Healing Research Center, Shi.C, Shiraz, Iran.
3- Department of Microbiology, Kaz.C, Islamic Azad University, Kazerun, Iran.
4- Department of Microbiology, Ja.C, Islamic Azad University, Jahrom, Iran. ,
Abstract: (640 Views)
This study investigated the anti-tumor properties of Lactobacillus delbrueckii, a probiotic bacterium, against HT-29 human colorectal cancer cells.
The primary objective was to evaluate the effects of Lactobacillus delbrueckii supernatant on gene expression, cell viability, apoptosis, and cell cycle progression in HT-29 cells. In Gilan Province, Iran, a native strain of Lactobacillus delbrueckii was identified from the supernatant of local yogurt. After treating HT-29 cells with different doses of the supernatant, the MTT test was used to calculate the IC50 value 24 hours later. Annexin V/PI staining and flow cytometry were used to evaluate the induction of apoptosis, and real-time PCR was used to measure changes in gene expression. The results showed that the supernatant of Lactobacillus delbrueckii strongly and dose-dependently reduced the growth of HT-29 cells. In addition to causing apoptosis and stopping the cell cycle in the G0/G1 phase, the therapy also increased the number of Sub-G1 cells, which is a sign of cell death. According to gene expression study, anti-apoptotic genes (AKT, Bcl-2) were downregulated while pro-apoptotic genes (PTEN, p53, and Bax) were upregulated. Interestingly, the natural isolate outperformed a conventional strain in terms of anticancer efficacy. These results demonstrate Lactobacillus delbrueckii's potential as a colorectal cancer treatment.
The primary objective was to evaluate the effects of Lactobacillus delbrueckii supernatant on gene expression, cell viability, apoptosis, and cell cycle progression in HT-29 cells. In Gilan Province, Iran, a native strain of Lactobacillus delbrueckii was identified from the supernatant of local yogurt. After treating HT-29 cells with different doses of the supernatant, the MTT test was used to calculate the IC50 value 24 hours later. Annexin V/PI staining and flow cytometry were used to evaluate the induction of apoptosis, and real-time PCR was used to measure changes in gene expression. The results showed that the supernatant of Lactobacillus delbrueckii strongly and dose-dependently reduced the growth of HT-29 cells. In addition to causing apoptosis and stopping the cell cycle in the G0/G1 phase, the therapy also increased the number of Sub-G1 cells, which is a sign of cell death. According to gene expression study, anti-apoptotic genes (AKT, Bcl-2) were downregulated while pro-apoptotic genes (PTEN, p53, and Bax) were upregulated. Interestingly, the natural isolate outperformed a conventional strain in terms of anticancer efficacy. These results demonstrate Lactobacillus delbrueckii's potential as a colorectal cancer treatment.
Keywords: Lactobacillus delbrueckii, anti-tumor properties, apoptosis, colorectal cancer, gene expression, cell cycle
Type of Study: Original Article |
Subject:
Cancer
Received: 2025/02/28 | Accepted: 2025/07/8 | Published: 2025/10/1
Received: 2025/02/28 | Accepted: 2025/07/8 | Published: 2025/10/1
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