International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
سه شنبه 1 مهر 1404
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Khoshkholgh Pahlaviani M R M, Emami A, Mahmoodi S M M, Rezaeian A A. Evaluation of properties of Lactobacillus delbrueckii as an antitumor agent: effects in cell cycle, apoptosis, and gene expression in HT-29 colorectal cancer cells. Int J Mol Cell Med 2025;
URL: http://ijmcmed.org/article-1-2558-fa.html
URL: http://ijmcmed.org/article-1-2558-fa.html
Evaluation of properties of Lactobacillus delbrueckii as an antitumor agent: effects in cell cycle, apoptosis, and gene expression in HT-29 colorectal cancer cells. مجله بین المللی سلولی و مولکولی. 1404;
: (1 مشاهده)
This study investigated the anti-tumor properties of Lactobacillus delbrueckii, a probiotic bacterium, against HT-29 human colorectal cancer cells.
The primary objective was to evaluate the effects of Lactobacillus delbrueckii supernatant on gene expression, cell viability, apoptosis, and cell cycle progression in HT-29 cells. In Gilan Province, Iran, a native strain of Lactobacillus delbrueckii was identified from the supernatant of local yogurt. After treating HT-29 cells with different doses of the supernatant, the MTT test was used to calculate the IC50 value 24 hours later. Annexin V/PI staining and flow cytometry were used to evaluate the induction of apoptosis, and real-time PCR was used to measure changes in gene expression. The results showed that the supernatant of Lactobacillus delbrueckii strongly and dose-dependently reduced the growth of HT-29 cells. In addition to causing apoptosis and stopping the cell cycle in the G0/G1 phase, the therapy also increased the number of Sub-G1 cells, which is a sign of cell death. According to gene expression study, anti-apoptotic genes (AKT, Bcl-2) were downregulated while pro-apoptotic genes (PTEN, p53, and Bax) were upregulated. Interestingly, the natural isolate outperformed a conventional strain in terms of anticancer efficacy. These results demonstrate Lactobacillus delbrueckii's potential as a colorectal cancer treatment.
The primary objective was to evaluate the effects of Lactobacillus delbrueckii supernatant on gene expression, cell viability, apoptosis, and cell cycle progression in HT-29 cells. In Gilan Province, Iran, a native strain of Lactobacillus delbrueckii was identified from the supernatant of local yogurt. After treating HT-29 cells with different doses of the supernatant, the MTT test was used to calculate the IC50 value 24 hours later. Annexin V/PI staining and flow cytometry were used to evaluate the induction of apoptosis, and real-time PCR was used to measure changes in gene expression. The results showed that the supernatant of Lactobacillus delbrueckii strongly and dose-dependently reduced the growth of HT-29 cells. In addition to causing apoptosis and stopping the cell cycle in the G0/G1 phase, the therapy also increased the number of Sub-G1 cells, which is a sign of cell death. According to gene expression study, anti-apoptotic genes (AKT, Bcl-2) were downregulated while pro-apoptotic genes (PTEN, p53, and Bax) were upregulated. Interestingly, the natural isolate outperformed a conventional strain in terms of anticancer efficacy. These results demonstrate Lactobacillus delbrueckii's potential as a colorectal cancer treatment.
نوع مطالعه: Original Article |
موضوع مقاله:
Cancer
دریافت: 1403/12/10 | پذیرش: 1404/4/17 | انتشار: 1404/5/6
دریافت: 1403/12/10 | پذیرش: 1404/4/17 | انتشار: 1404/5/6
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