International Journal of Molecular and Cellular Medicine (IJMCM)
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences
Fri, May 9, 2025
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Volume 13, Issue 4 (Int J Mol Cell Med 2024)
Int J Mol Cell Med 2024, 13(4): 404-416 |
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Rezaei Khozani N, Shayesteh Pour M, Yekani M, Hejazi S H, Saffari M. Anti-tumor Effects of Recombinant Clostridium α-Toxin on Breast Cancer: An In Vitro and In Vivo Study. Int J Mol Cell Med 2024; 13 (4) :404-416
URL: http://ijmcmed.org/article-1-2403-en.html
URL: http://ijmcmed.org/article-1-2403-en.html
Nahid Rezaei Khozani1 
, Mohammad Shayesteh Pour2 , Mina Yekani3 , Seyed Hossein Hejazi4 , Mahmood Saffari5
, Mohammad Shayesteh Pour2 , Mina Yekani3 , Seyed Hossein Hejazi4 , Mahmood Saffari5
1- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran. , nahidrezaei653@gmail.com
2- Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
2- Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
3- Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
4- Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
5- Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Abstract: (542 Views)
Cancer is the second leading cause of death worldwide, surpassed only by cardiovascular diseases. This study investigated the anticancer effects of recombinant Clostridium α-toxin on breast cancer, both in vitro and in vivo. The entire coding sequence of a codon-optimized α-toxin was designed, cloned into the pET28a (+) vector, and expressed as recombinant α-toxin in Escherichia coli (E. coli) BL 21(DE3) cells transformed with the recombinant plasmid. The recombinant α-toxin was purified using Ni²⁺ affinity chromatography, and its accuracy and purity were confirmed through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The anticancer effects of purified α-toxin were then assessed in vitro and animal models against MCF-7 breast cancer cells. Protein analysis confirmed the presence of a 48 kDa band corresponding to the recombinant α-toxin. Additionally, the IC₅₀ values of α-toxin against MCF-7 cells at 24, 48, and 72 h were 407.3±2.392 μg/mL, 287.3±5.411 μg/mL, and 258.1±4.671 μg/mL, respectively. In vivo, results demonstrated a significant reduction in mean cancer nodule size following α-toxin treatment (p<0.001). These findings suggest that α-toxin may serve as a promising candidate for breast cancer therapy.
Type of Study: Original Article |
Subject:
Cancer
Received: 2024/08/8 | Accepted: 2024/10/23 | Published: 2024/12/4
Received: 2024/08/8 | Accepted: 2024/10/23 | Published: 2024/12/4
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