دوره 13، شماره 4 - ( 7-1403 )                   | برگشت به فهرست نسخه ها

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Rezaei Khozani N, Shayesteh Pour M, Yekani M, Hejazi S H, Saffari M. Anti-tumor Effects of Recombinant Clostridium α-Toxin on Breast Cancer: An In Vitro and In Vivo Study. Int J Mol Cell Med 2024; 13 (4) :404-416
URL: http://ijmcmed.org/article-1-2403-fa.html
Anti-tumor Effects of Recombinant Clostridium α-Toxin on Breast Cancer: An In Vitro and In Vivo Study. مجله بین المللی سلولی و مولکولی. 1403; 13 (4) :404-416

URL: http://ijmcmed.org/article-1-2403-fa.html

Anti-tumor Effects of Recombinant Clostridium α-Toxin on Breast Cancer: An In Vitro and In Vivo Study
:   (701 مشاهده)
Cancer is the second leading cause of death worldwide, surpassed only by cardiovascular diseases. This study investigated the anticancer effects of recombinant Clostridium α-toxin on breast cancer, both in vitro and in vivo. The entire coding sequence of a codon-optimized α-toxin was designed, cloned into the pET28a (+) vector, and expressed as recombinant α-toxin in Escherichia coli (E. coli) BL 21(DE3) cells transformed with the recombinant plasmid. The recombinant α-toxin was purified using Ni²⁺ affinity chromatography, and its accuracy and purity were confirmed through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The anticancer effects of purified α-toxin were then assessed in vitro and animal models against MCF-7 breast cancer cells. Protein analysis confirmed the presence of a 48 kDa band corresponding to the recombinant α-toxin. Additionally, the IC₅₀ values of α-toxin against MCF-7 cells at 24, 48, and 72 h were 407.3±2.392 μg/mL, 287.3±5.411 μg/mL, and 258.1±4.671 μg/mL, respectively. In vivo, results demonstrated a significant reduction in mean cancer nodule size following α-toxin treatment (p<0.001). These findings suggest that α-toxin may serve as a promising candidate for breast cancer therapy.
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نوع مطالعه: Original Article | موضوع مقاله: Cancer
دریافت: 1403/5/18 | پذیرش: 1403/8/2 | انتشار: 1403/9/14
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