Effects of hsa-piR-32877 Suppression with Antisense LNA GapmeRs on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells

Naseri, Sepideh; Sharifi, Mohammadreza; Mehrzad, Valiollah

doi:10.22088/IJMCM.BUMS.12.1.18

Volume 12, Issue 1 (Int J Mol Cell Med 2023) Int J Mol Cell Med 2023, 12(1): 18-29 | Back to browse issues page

‎ 10.22088/IJMCM.BUMS.12.1.18

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Naseri S, Sharifi M, Mehrzad V. Effects of hsa-piR-32877 Suppression with Antisense LNA GapmeRs on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells. Int J Mol Cell Med 2023; 12 (1) :18-29
URL: http://ijmcmed.org/article-1-2115-en.html

Effects of hsa-piR-32877 Suppression with Antisense LNA GapmeRs on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells

Sepideh Naseri¹

, Mohammadreza Sharifi²

, Valiollah Mehrzad³

1- Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
2- Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. , mo_sharifi@med.mui.ac.ir
3- Department of Internal Medicine, Division of Hematology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract: (1452 Views)

Acute myeloid leukemia (AML) is an invasive form of hematologic malignancies which results in the overproduction of myeloid cells in the bone marrow. Aberrant expression of piwi-interacting RNAs (piRNAs) which belong to small non-coding RNAs, play important roles in different cancer cells' progress. hsa- piR- 32877 is up-regulated in AML. Down regulation of hsa-piR-32877 by antisense LNA GapmeRs could be potential for suppression of myeloid cell proliferation and induce myeloid cell apoptosis. We have blocked the expression of hsa-piR-32877 by antisense LNA GapmeRs in human bone marrow blast cells, and the M-07e cell line. Samples were transfected with antisense LNA GapmeRs at 24, 48, and 72 hours. The Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to investigate the expression of hsa-piR-32877, CASP3, and CASP9. Both CASP3 and CASP9 play important roles in apoptosis. Cell proliferation was studied via CFSE (carboxyfluorescein diacetate succinimidyl ester) assay. Results showed that hsa-piR-32877 was down-regulated by antisense LNA GapmeRs in the patient and cell line samples. Also, after transfection, cell proliferation and apoptosis decreased and increased, respectively. Our data suggested that hsa-piR-32877 suppression may act as a novel therapeutic method for the inhibition of human leukemic cells proliferation in AML.

Keywords: Acute myeloid leukemia, Antisense LNA GapmeRs, hsa-piR-32877, CASP3, CASP9

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Type of Study: Original Article | Subject: Hematology
Received: 2023/02/18 | Accepted: 2023/09/17 | Published: 2023/09/19