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Expression Assessment of the Helicobacter pylori babA and sabA Genes in Patients with Peptic Ulcer, Duodenal Ulcer and Gastric Cancer
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Javad Shokri Shirvani1    , Maryam Salehi2 , Amirmohammad Rezaei Majd3 , Farzin Sadeghi4 , Elaheh Ferdosi-Shahandashti1 , Soraya Khafri5 , Mehdi Rajabnia 6 |
1- Biomedical and Microbial Advanced Technologies Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
2- Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
3- Razi Hospital. Tehran University of Medical Sciences, Tehran, Iran.
4- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
5- Infertility and Reproductive Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
6- Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. , ramazan69@yahoo.com |
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Abstract: (476 Views) |
Helicobacter pylori as a common gastrointestinal (GI) pathogen must possess certain virulence characteristics to colonize the stomach, evade host immune responses, and subsequently induce GI diseases. This research aimed to investigate the expression level of two important genes, the sialic acid-binding adherence (SabA) and the blood group antigen-binding adhesion (BabA) in H. pylori strains isolated from adult patients living in the northern part of Iran, and their association with peptic ulcer disease (PUD) and gastric cancer (GC). This cross-sectional study was carried out on adult patients referring to the GI clinic of the hospitals affiliated to Babol University of Medical Sciences, Iran. New cases diagnosed with gastritis, peptic ulcer or gastric cancer were included. Endoscopic-guided gastric biopsies were examined and H. pylori positive colonies were analyzed to determine the expression of babA and sabA genes, utilizing specific primers and the SYBR Green dye. Among 175 patients with mean age of 51.6±15.6 years, 101 (57.7%) of the individuals tested positive for H. pylori infection. Statistical analysis revealed a significant correlation between sabA (P=0.003) and babA (P=0.002) gene expression and development of PUD and GC. Smoking (P=0.052), gender (P=0.004) and positive babA gene expression (P=0.009) had the greatest association with occurrence of PUD or GC in H. pylori positive patients. In summary, the presence of the sabA gene in people infected with H. pylori increased the risk of GC compared to gastritis, while, the presence of the babA gene was significantly increased in gastric ulcer patients. Considering the diversity of H. pylori isolates and the varying results observed in different geographical regions, further comprehensive studies are required to evaluate the function of these genes in H. pylori pathogenesis and their relationship with clinical outcomes.
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| Keywords: Helicobacter pylori, Peptic Ulcer Disease, Gastric Cancer, sabA, babA |
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Full-Text [PDF 273 kb]
(280 Downloads)
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Type of Study: Original Article |
Subject:
Infectious disease (Molecular and Cellular aspects)
Received: 2023/06/14 | Accepted: 2023/11/25 | Published: 2023/12/16
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