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:: Volume 7, Issue 2 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(2): 91-101 Back to browse issues page
Potential Use of Amniotic Membrane Derived Scaffold for Cerebrospinal Fluid Applications
Fereshteh Dorazehi 1, Mohammad Nabiuni * 2, Hanieh Jalali 3
1- Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
2- Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran. , devbiokharazmi@gmail.com
3- Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Abstract:   (285 Views)
Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic membrane (AM). BM-MSCs were collected from femurs and tibias, and were cultured in Dulbecco's Modified Eagle's Medium. The placenta was harvested from healthy women during cesarean section and AM was acellularized using EDTA and physical scrubbing. e-CSF was harvested from rat fetuses at E17. Adequate numbers of BM-MSCs were cultured on acellularized membrane, and were treated with E17 CSF for 7 days. MTT (3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay confirmed the survival and proliferation of BM-MSCs cultured on AM derived scaffold. Hematoxylin/eosin staining and scanning electron microscopy showed the morphological and the structural changes of BM-MSCs throughout the culture and treatment with e-CSF. The results of immunocytochemistry showed that microtubule associated protein 2 and beta-III tubulin were expressed in BM-MSCs cultured on acellular amnion scaffold and treated with e-CSF. Our results showed for the first time that the combination of acellular AM as a natural scaffold and e-CSF as a source of neurological factors could effectively improve the BM-MSCs cultivation and differentiation.
Keywords: Cerebrospinal Fluid, Bone Marrow Mesenchymal Stem Cells, Neural Differentiation, Amnion Membrane Derived Scaffold
Full-Text [PDF 517 kb]   (60 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2018/04/8 | Accepted: 2018/08/14 | Published: 2018/09/11
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Dorazehi F, Nabiuni M, Jalali H. Potential Use of Amniotic Membrane Derived Scaffold for Cerebrospinal Fluid Applications. Int J Mol Cell Med. 2018; 7 (2) :91-101
URL: http://ijmcmed.org/article-1-843-en.html


Volume 7, Issue 2 (Int J Mol Cell Med 2018) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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