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:: Volume 2, Issue 1 (Int J Mol Cell Med 2013) ::
Int J Mol Cell Med 2013, 2(1): 9-13 Back to browse issues page
Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
Seyed Mahmoud Amin Marashi 1, Ramazan Rajabnia 2, Abbas Ali Imani Fooladi 3, Zohreh Hojati 4, Sharareh Moghim 5, Bahram Nasr Esfahani * 6
1- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
2- Infectious Diseases & Tropical Medicine Research Center, Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran.
3- Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
4- Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
5- Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran.
6- Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran. , Nasr@hlth.mui.ac.ir
Abstract:   (7163 Views)
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain.
Keywords: Vibrio cholerae, RT-qPCR, ctxAB expression
Full-Text [PDF 128 kb]   (1972 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2013/03/5 | Accepted: 2013/06/22 | Published: 2013/06/22
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Amin Marashi S M, Rajabnia R, Imani Fooladi A A, Hojati Z, Moghim S, Nasr Esfahani B. Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR. Int J Mol Cell Med. 2013; 2 (1) :9-13
URL: http://ijmcmed.org/article-1-73-en.html


Volume 2, Issue 1 (Int J Mol Cell Med 2013) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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