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:: Volume 6, Issue 3 (Int J Mol Cell Med (In press) 2017) ::
Int J Mol Cell Med 2017, 6(3): 0-0 Back to browse issues page
Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from Enteroinvasive Escherichia coli (EIEC) Invasion
Zohreh Khodaii 1, Sayyed Mohammad Hossein Ghaderian 2, Mahboobeh Mehrabani Natanzi *3
1- Postdoc Dietary supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran., Dietary supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
2- Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Dietary supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran., Dietary supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran. , mmehrabani95@gmail.com
Abstract:   (519 Views)
Probiotic microorganisms have attracted a growing interest for prevention and therapy of gastrointestinal disorders. Many probiotic strains have been shown to inhibit growth and metabolic activity of enteropathogenic bacteria as well as their adhesion and invasion to intestinal cells. In the present study, we evaluated the interference of bacteria-free supernatants (BFS) of cultures belonging to sixteen strains of lactobacilli and bifidobacteria, with invasion of enteroinvasive Escherichia coli (EIEC) strain, by using human colonic adenocarcinoma cell lines, T84 and Caco2 cells. To assess invasion of Caco-2 and T84 cells by EIEC, and measure the number of pathogens inside the enterocytes, the gentamicin protection assay was conducted. In addition, three different invasion inhibition assays were designed; namely co-incubation, pre-incubation and treatment with the BFS of probiotics. Data obtained and theoretical calculation showed that the most effective assay in the prevention of pathogen invasion was treatment with BFS. Besides, co-incubation assay was more valid than pre-incubation assay in invasion prevention. The obtained results suggest that probiotics may produce some metabolites that strongly prevent invasion of enteroinvasive E.coli into the small and large intestine. Also, probiotics are able to compete with or exclude pathogen invasion.
Keywords: Probiotics, enteroinvasive Escherichia coli, Caco-2 cells, T84 cells, adhesion
Full-Text [PDF 221 kb]   (151 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2017/08/1 | Accepted: 2017/09/11 | Published: 2017/09/26
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Khodaii Z, Ghaderian S M H, Mehrabani Natanzi M. Probiotic Bacteria and their Supernatants Protect Enterocyte Cell Lines from Enteroinvasive Escherichia coli (EIEC) Invasion. Int J Mol Cell Med. 2017; 6 (3)
URL: http://ijmcmed.org/article-1-722-en.html
Volume 6, Issue 3 (Int J Mol Cell Med (In press) 2017) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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