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:: Volume 5, Issue 2 (Int J Mol Cell Med 2016) ::
Int J Mol Cell Med 2016, 5(2): 106-113 Back to browse issues page
Expression of HCA2 Receptors in Femoral Epiphysis and Metaphysis of Rats with Dexamethasone-Induced Osteoporosis
Tahoora Shomali 1, Mohammad Kamalpour2, Mehdi Fazeli2, Alireza Rafati2
1- Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. , tshomali@shirazu.ac.ir
2- Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Abstract:   (4760 Views)

The present study describes the changes in expression of hydroxy- carboxylic acid receptor 2 (HCA2 receptor) in femoral epiphysis and metaphysis of rats with glucocorticoid-induced osteoporosis (GIO). 16 growing male Sprauge dawley rats were randomly divided into two equal groups consisting of normal control and rats that were rendered osteoporotic by receiving 0.1 mg/kg/day dexamethasone through injected subcutaneously. After 4 weeks, all rats were sacrificed and immediately right and left femoral bones were removed for RT-qPCR and histological examination, respectively. Immunohistochemical parameters using a primary rabbit polyclonal GPR109A antibody in hematoxylin and eosin- counter stained slides were determined. HCA2 receptor expression was evaluated using RT- qPCR. Qualitative and histomorphometric evaluation of slides revealed the establishment of glucocorticoid- induced osteoporosis (GIO) in rats treated with dexamethasone. In immunohistochemical study, dexamethasone administration appreciably reduced receptor density in all evaluated cell types and in total slides as compared to control. mRNA level of HCA2 receptor gene was reduced in dexamethasone- treated group. GIO may be associated with down regulation of HCA2 receptors in proximal femoral bone of rats at mRNA as well as protein level in no- cell type-specific manner, although reduction in protein expression needs to be further confirmed by western blotting.

Keywords: Glucocorticoid-induced osteoporosis, hydroxyl-carboxylic acid receptor 2, RT-qPCR, immunohistochemistry, rat
Full-Text [PDF 206 kb]   (1584 Downloads)    
Type of Study: Original Article | Subject: Histopathology
Received: 2016/04/11 | Accepted: 2016/06/11 | Published: 2016/06/19
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Shomali T, Kamalpour M, Fazeli M, Rafati A. Expression of HCA2 Receptors in Femoral Epiphysis and Metaphysis of Rats with Dexamethasone-Induced Osteoporosis. Int J Mol Cell Med. 2016; 5 (2) :106-113
URL: http://ijmcmed.org/article-1-500-en.html


Volume 5, Issue 2 (Int J Mol Cell Med 2016) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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