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:: Volume 5, Issue 2 (Int J Mol Cell Med 2016) ::
Int J Mol Cell Med 2016, 5(2): 80-89 Back to browse issues page
Carcinoembryonic Antigen Expression and Resistance to Radiation-and 5-Fluorouracil-Induced Apoptosis and Autophagy
Ebrahim Eftekhar1, Hajar Jaberi1, Fakhraddin Naghibalhossaini 2
1- Department of Biochemistry, Shiraz University of Medical Sciences, School of Medicine, Shiraz, Iran.
2- Autoimmune Research Center, Shiraz University of Medical Sciences, School of Medicine, Shiraz, Iran. , fakhraddin.naghibalhossaini@mail.mcgill.ca
Abstract:   (5018 Views)

Understanding the mechanism of tumor resistance is critical for cancer therapy. In this study, we investigated the effect of carcinoembryonic antigen (CEA) overexpression on UV-and 5-fluorouracil (5-FU)-induced apoptosis and autophagy in colorectal cancer cells. We used histone deacetylase (HDAC) inhibitor, NaB and DNA demethylating agent, 5- azacytidine (5-AZA) to induce CEA expression in HT29/219 and SW742 colorectal cancer cell lines. MTT assay was used to measure IC50 value of the cells exposed to graded concentrations of 5- FU with either 0.1 mM NaB or 1 μM 5-AZA for 72 h . Using CHO- and SW742-CEA transfectants, we also investigated the effect of CEA expression on UV- and 5-FU-induced apoptosis and autophagy. Treatment of HT29/219 cell line with NaB and 5-AZA increased CEA expression by 29% and 31%, respectively. Compared with control cells, the IC50 value for 5-FU of NaB and 5-AZA-treated cells increased by 40% and 57%, respectively. Treatment of SW742 cells with NaB or 5-AZA increased neither CEA expression nor the IC50 value for 5-FU. In comparison to parental cells, CEA expression also significantly protected transfected cells against UV-induced apoptosis. Decreased proportions of autophagy and apoptosis were also observed in 5-FU treated SW742- and CHO-CEA transfectants. We conclude that CEA expression can effectively protect colorectal cancer cells against radiation and drug-induced apoptosis and autophagy.

Keywords: Carcinoembryonic antigen (CEA), colorectal cancer, 5-fluorouracil, apoptosis, autophagy
Full-Text [PDF 254 kb]   (1828 Downloads)    
Type of Study: Original Article | Subject: Biomarkers (diagnosis & treatment)
Received: 2016/01/29 | Accepted: 2016/04/16 | Published: 2016/05/17
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Eftekhar E, Jaberi H, Naghibalhossaini F. Carcinoembryonic Antigen Expression and Resistance to Radiation-and 5-Fluorouracil-Induced Apoptosis and Autophagy. Int J Mol Cell Med. 2016; 5 (2) :80-89
URL: http://ijmcmed.org/article-1-460-en.html


Volume 5, Issue 2 (Int J Mol Cell Med 2016) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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