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:: Volume 4, Issue 4 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(4): 227-234 Back to browse issues page
Association between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients
Heshmat Shahi1, Somayeh Reiisi2, Rasol Bahreini3, Nader Bagheri1, Loghman Salimzadeh1, Hedayatollah Shirzad 4
1- Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2- Department of Genetic, Faculty of basic science, Shahrekoed University, Shahrekord, Iran
3- Department of Internal Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
4- Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. , shirzadeh@yahoo.com
Abstract:   (5156 Views)

Helicobacter pylori (H. pylori) infection has been reported in more than half of the world human population. It is associated with gastric inflammation and noticeable infiltration of the immune cells to the stomach mucosa by several cytokines secretion. IL-1&beta, IL-18 have been shown to contribute to H. pylori induced gastritis, but the details of inflammation and association of virulence factors remain unclear. IL-1 cytokine family has a new additional cytokine, Interleukin-33 (IL-33), which is contemplated to have an important role for host defense against microorganisms. H. pylori virulence factors important in gastritis risk are the cag pathogenicity island (cag-PAI) and babA. This study evaluated IL-33 mucosal mRNA expression levels in infected and uninfected patients and its relationship with bacterial virulence factors cagA, babA2 and type of gastritis. Total RNA was extracted from gastric biopsies of 79 H. pylori-infected patients and 51 H. pylori-negative patients. Mucosal IL-33 mRNA expression levels in gastric biopsies were assessed using real-time PCR. Existence of virulence factors were detected by PCR. IL-33 mRNA expression was significantly higher in biopsies of H. pylori-infected patients compared to H. pylori-uninfected patients (P<0.0001). Also there was a direct relationship between virulence factor bab-A2 and enhancement in IL-33 mRNA expression. Furthermore, IL-33 mRNA expression level was significantly lower in chronic gastritis patients compared with patients with active gastritis (P<0.001). IL-33 may play a crucial role in the inflammatory response and induction of the chronic gastritis and severity of inflammatory changes in the gastric mucosa.

Keywords: Helicobacter pylori, gastritis, interleukin-33, virulence factor
Full-Text [PDF 125 kb]   (2322 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2015/11/25 | Accepted: 2015/12/7 | Published: 2015/12/23
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Shahi H, Reiisi S, Bahreini R, Bagheri N, Salimzadeh L, Shirzad H. Association between Helicobacter pylori cagA, babA2 Virulence Factors and Gastric Mucosal Interleukin-33 mRNA Expression and Clinical Outcomes in Dyspeptic Patients. Int J Mol Cell Med. 2015; 4 (4) :227-234
URL: http://ijmcmed.org/article-1-423-en.html


Volume 4, Issue 4 (Int J Mol Cell Med 2015) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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