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:: Volume 4, Issue 3 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(3): 167-173 Back to browse issues page
The Frequency of Exotoxin A and Exoenzymes S and U Genes Among Clinical Isolates of Pseudomonas aeruginosa in Shiraz, Iran
Arshid Yousefi-Avarvand 1, Reza Khashei * 2, Hadi Sedigh Ebrahim-Saraie 1, Amir Emami 1, Kamiar Zomorodian 3, Mohammad Motamedifar 1
1- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
2- Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. , khasheir@sums.ac.ir
3- Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Abstract:   (3876 Views)

Pseudomonas aeruginosa as an opportunistic pathogen produces several virulence factors. The most important of these factors are exotoxin A and type III secretion system (T3SS). The aim of this study was to determine the frequency of toxA, exoU and exoS genes among clinical isolates of P. aeruginosa. In this cross-sectional study from September 2011 to February 2012, 156 P. aeruginosa isolates were recovered from different clinical samples. Susceptibility testing against 10 antibiotics was performed on individual isolates by the disc diffusion method according to CLSI guidelines. Extracted DNA was subjected to PCR assay for determining the presence of toxA, exoU and exoS genes. Overall, the frequency of toxA, exoU and exoS genes were 90.4%, 66.7% and 65.4%, respectively. All of the abdominal and eye isolates were exoS+. The frequency of exoS+/exoU- and exoS-/exoU+ genotypes was estimated 19.2% and 16.2%, respectively. Indeed, genotypes exoS+/exoU+ and exoS-/exoU- were found with frequencies of 48.7% and 15.3%, respectively. The highest and lowest antibiotic resistance rate was seen against azteroenam (94.2%) and amikacin (44.9%), respectively. Fluoroqinolone-resistant isolates were isolated with frequency of 45.8%. Multi-drug resistant (MDR) isolates were detected in 62.8% of isolates. The resistance rate in exoU+ isolates was 86% compared to 66% in exoS+ isolates. The high frequencies of virulence genes detected in our clinical isolates with notable antibiotic resistance rates indicate the potential risk of these isolates in nosocomial infections.

Keywords: Pseudomonas aeruginosa, exotoxin A, exoenzyme S, exoenzyme U
Full-Text [PDF 92 kb]   (1485 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2015/06/5 | Accepted: 2015/07/20 | Published: 2015/08/25
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Yousefi-Avarvand A, Khashei R, Sedigh Ebrahim-Saraie H, Emami A, Zomorodian K, Motamedifar M. The Frequency of Exotoxin A and Exoenzymes S and U Genes Among Clinical Isolates of Pseudomonas aeruginosa in Shiraz, Iran. Int J Mol Cell Med. 2015; 4 (3) :167-173
URL: http://ijmcmed.org/article-1-337-en.html


Volume 4, Issue 3 (Int J Mol Cell Med 2015) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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