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Anti-influenza Activity of a Novel Polyoxometalate Derivative (POM-4960)
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Seyed Masoud Hosseini1   , Elham Amini 2, Masoumeh Tavassoti Kheiri3 , Parvaneh Mehrbod3 , Mahsa Shahidi3 , Ebrahim Zabihi4 |
1- Department of Microbiology, Faculty of Biological Science, Shahid Beheshti University, Evin, Tehran, Iran.
2- Influenza Unit, Pasteur Institute of IRAN, 12 Farvardin St. Tehran, Iran. , elham.amini@gmail.com
3- Influenza Unit, Pasteur Institute of IRAN, 12 Farvardin St. Tehran, Iran.
4- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. |
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Abstract: (16682 Views) |
| There are many effective chemothereutic agents used in influenza disease which some of them inhibit virus replication by interfering with FluV (influenza virus) viral binding or its penetration into cell membrane. A series of polyoxometalates compounds such as POM-523 and PM-504 have been synthesized and have showed inhibitory effects on viruses. In this study we examined anti influenza activity of a novel polyoxometalate derivative (POM-4960) synthesized in the Faculty of Chemistry of Damghan University of Basic Sciences.To evaluate the anti-influenza activity of POM, following the treatment of FluV with POM at different temperatures and incubation periods, viral titer reduction was assessed by haemaglutination assay (HA). The 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine TCID50 (tissue culture infective dose) of virus, CC50 (median cytotoxic concentration) of POM, protection percentage and antiviral activity of POM in cell culture. RT-PCR and direct Immunofluorescent assays were performed to evaluate the effect of POM on viral infection and viral RNA load, respectively.
POM reduced HA titer near to zero in all cell culture specimens and showed high protection against viral infection of the cells. Reduction in viral infection was confirmed by RT-PCR and Immunofluorescent staining methods. Moreover, this POM derivative has a dual (cumulative) effect on attachment and penetration inhibition compared to other POM’s with just one inhibitory effect. POM-4960 could be considered as a powerful anti-influenza agent with low toxicity and high antiviral potency. |
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| Keywords: POM, influenza virus, RT-PCR, immunofluorescent, MTT |
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Full-Text [PDF 157 kb]
(5148 Downloads)
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Type of Study: Original Article |
Subject:
Infectious disease (Molecular and Cellular aspects)
Received: 2012/01/1 | Accepted: 2013/09/14 | Published: 2013/09/14
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