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:: Volume 4, Issue 2 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(2): 120-127 Back to browse issues page
Multiple Inherited Thrombophilic Gene Polymorphisms in Spontaneous Abortions in Turkish Population
Sinem Yalcintepe 1, Ozturk Ozdemir2, Servet Ozden Hacivelioglu3, Cisem Akurut2, Evrim Koc3, Ahmet Uludag2, Emine Cosar3, Fatma Silan2
1- Department of Medical Genetics, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey. , sinem_atik@yahoo.com
2- Department of Medical Genetics, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
3- Department of Gynecology and Obstetrics, School of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey.
Abstract:   (5198 Views)
The aim of this study was to investigate the possible role of multiple inherited thrombophilic gene variations in women with unexplained spontaneous abortions. For this purpose, the Factor V Leiden (FVL) (rs6025), Prothrombin G20210A (rs1799963), MTHFR C677T (rs1801133), PAI-1 4G/5G (rs1799889), ACE I/D (rs1799752), eNOS E298D (rs1799983), and Apo E E2/E3/E4 (rs429358) polymorphisms were genotyped and correlated in spontaneously aborted fetal materials, their mothers and fertile women. Twenty three abortion materials, 22 women with ≥1 unexplained fetal loss, and 22 control subjects with at least two healthy term infants as a control group were studied. Target SNPs for each gene were analyzed by real time-PCR technique after genomic DNA isolation from maternal blood-EDTA, control group blood-EDTA and spontaneously aborted fetal tissues. Some cases had a single thrombophilic polymorphism, but the rest of the patients and fetal materials had combined thrombophilic polymorphisms. The PAI-1 4G/5G+4G/4G (P= 0.0017), 4G/4G (P= 0.0253), eNOS 894GT+894TT (P=0.0011) genotypes and T allele (P=0.0185), Apo E E3/E4+E3/E2+E2/E4 (P<0.0001) genotypes, E2 (P<0.0001) and E4 (P<0.0001) alleles were higher in spontaneously aborted fetal materials when compared to their mothers and control group. The Factor V Leiden rs6025, Prothrombin G20210A, MTHFR C677T, ACE I/D genotypes were different for each group but not statistically significant due to relatively small size of the samples (P>0.05). Our results indicated that combined thrombophilic gene variations may be associated with increased risk for spontaneous abortions and results need to be confirmed by larger sample size.
Keywords: Spontaneous abortion, thrombophilia, polymorphism, fetus
Full-Text [PDF 90 kb]   (1976 Downloads)    
Type of Study: Original Article | Subject: Genetics & Disease
Received: 2015/01/16 | Accepted: 2015/03/1 | Published: 2015/05/9
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Yalcintepe S, Ozdemir O, Hacivelioglu S O, Akurut C, Koc E, Uludag A, et al . Multiple Inherited Thrombophilic Gene Polymorphisms in Spontaneous Abortions in Turkish Population. Int J Mol Cell Med. 2015; 4 (2) :120-127
URL: http://ijmcmed.org/article-1-284-en.html


Volume 4, Issue 2 (Int J Mol Cell Med 2015) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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