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:: Volume 4, Issue 1 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(1): 54-59 Back to browse issues page
RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?
Ali Akbar Samadani1, Novin Nikbakhsh2, Sadegh Fatahi1, Seyed Mohsen Aghajanpour Mir1, Seyedeh Narges Mosavi Kani1, Rogheyeh Pourbagher1, Zeynab Abediyan1, Haleh Akhavan Niaki 3
1- Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
2- Department of Surgery, Babol university of Medical Sciences, Babol, Iran.
3- Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran. , Halehakhavan@yahoo.com
Abstract:   (5172 Views)
The reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was accomplished in order to optimize the RNA extraction methods and compare the tissue amounts or tissues quality. Relatively, the cancerous tissues of human's stomach in fresh and frozen conditions and also the mouse's fresh tissue were studied. Some factors like the amount of samples, efficacy differences of diverse extraction's buffers (TriPure, Trizol) and also the efficacy of b-mercaptoethanol were compared and investigated. The results indicated that the less amount (1-2 mg) compared to other amounts (2-5 mg, 5-15 mg) yielded the best quality and the RNA's bands (5S, 18S, 28S) were observed perfectly. Relatively, comparing and measuring some kinds of buffers (Trizol, TriPure) indicated no difference in RNA extraction quality. The last investigated factor was the effect of b- mercaptoethanol which was used along with TriPure in order to remove the RNAse. Conclusively, no effective impression was observed.
Keywords: RNA extraction, frozen tissue, cancerous tissues
Full-Text [PDF 119 kb]   (1692 Downloads)    
Type of Study: Original Article | Subject: Medical Pharmacology
Received: 2015/01/4 | Accepted: 2015/01/26 | Published: 2015/02/2
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Samadani A A, Nikbakhsh N, Fatahi S, Aghajanpour Mir S M, Mosavi Kani S N, Pourbagher R, et al . RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?. Int J Mol Cell Med. 2015; 4 (1) :54-59
URL: http://ijmcmed.org/article-1-280-en.html


Volume 4, Issue 1 (Int J Mol Cell Med 2015) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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