International Journal of Molecular and Cellular Medicine (IJMCM)
Babol University of Medical Sciences
Mon, Oct 27, 2025
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Volume 14, Issue 3 (Int J Mol Cell Med 2025)
Int J Mol Cell Med 2025, 14(3): 843-855 |
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Torkashvand S, Kazemi S, Mansoori R, Moghadamnia A A, Ashrafpour M. Melatonin Ameliorates 5-Fluorouracil-Induced Cytotoxicity and Apoptosis in H9c2 Cell Line: Insights into Cytoprotection and Anti-Apoptotic Mechanisms. Int J Mol Cell Med 2025; 14 (3) :843-855
URL: http://ijmcmed.org/article-1-2583-en.html
URL: http://ijmcmed.org/article-1-2583-en.html
Sama Torkashvand1 
, Sohrab Kazemi2 , Razieh Mansoori1 , Ali Akbar Moghadamnia3 , Manouchehr Ashrafpour4
, Sohrab Kazemi2 , Razieh Mansoori1 , Ali Akbar Moghadamnia3 , Manouchehr Ashrafpour4
1- Student Research Committee, Health Research Institute, Babol, Iran
2- Cellular and Molecular Biology Research Center, Health Research Institute, Babol, Iran
3- Pharmaceutical Sciences Research Center, Health Research Institute, Babol, Iran
4- Mobility Impairment Research Center, Health Research Institute, Babol, Iran ,mnrashrafpour@yahoo.com
2- Cellular and Molecular Biology Research Center, Health Research Institute, Babol, Iran
3- Pharmaceutical Sciences Research Center, Health Research Institute, Babol, Iran
4- Mobility Impairment Research Center, Health Research Institute, Babol, Iran ,
Abstract: (254 Views)
Cardiotoxicity represents a significant adverse effect associated with 5-fluorouracil (5-FU), a widely used chemotherapeutic agent. Melatonin (MLT), a powerful antioxidant and agent that prevents apoptosis, has shown promise in mitigating various toxicities. This study evaluated the cardioprotective effect of MLT on 5-FU-induced cardiotoxicity (5-FU-IC) in the H9c2 cardiomyoblast cell line.
The cells were grown in DMEM + FBS and divided into four groups: control (untreated), 5-FU-treated (varying concentrations for 48 hours), MLT-treated (varying concentrations), and 5-FU plus MLT-treated (combined treatment for 48 hours). The cell viability was evaluated using the MTT assay, while apoptosis was analyzed through flow cytometry following Annexin V staining and caspase-3/7 (Cas-3/7) activity assays.
Treatment with 5-FU led to a significant decrease in the viability of H9c2 cells in a dose-dependent fashion, with an estimated IC50 value of 400 μM. Co-treatment with MLT at 100 and 200 μM significantly enhanced cell viability and reduced apoptosis induced by 5-FU, as demonstrated by flow Cytometry and reduced Cas-3/7 activity. These results emphasize the protective effects of MLT against 5-FU-IC, primarily through its anti-apoptotic mechanisms.
These findings underscore the importance of MLT to protect against 5-FU-IC through its anti-apoptotic properties. MLT shows promise as a cardioprotective agent in mitigating 5-FU-IC, providing perspectives on its potential therapeutic application in mitigating cardiac risks linked to chemotherapy.
The cells were grown in DMEM + FBS and divided into four groups: control (untreated), 5-FU-treated (varying concentrations for 48 hours), MLT-treated (varying concentrations), and 5-FU plus MLT-treated (combined treatment for 48 hours). The cell viability was evaluated using the MTT assay, while apoptosis was analyzed through flow cytometry following Annexin V staining and caspase-3/7 (Cas-3/7) activity assays.
Treatment with 5-FU led to a significant decrease in the viability of H9c2 cells in a dose-dependent fashion, with an estimated IC50 value of 400 μM. Co-treatment with MLT at 100 and 200 μM significantly enhanced cell viability and reduced apoptosis induced by 5-FU, as demonstrated by flow Cytometry and reduced Cas-3/7 activity. These results emphasize the protective effects of MLT against 5-FU-IC, primarily through its anti-apoptotic mechanisms.
These findings underscore the importance of MLT to protect against 5-FU-IC through its anti-apoptotic properties. MLT shows promise as a cardioprotective agent in mitigating 5-FU-IC, providing perspectives on its potential therapeutic application in mitigating cardiac risks linked to chemotherapy.
Type of Study: Original Article |
Subject:
Cell Biology
Received: 2025/06/19 | Accepted: 2025/09/21 | Published: 2025/10/1
Received: 2025/06/19 | Accepted: 2025/09/21 | Published: 2025/10/1
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