International Journal of Molecular and Cellular Medicine (IJMCM)
Babol University of Medical Sciences
Tue, Nov 4, 2025
[Archive]
Volume 14, Issue 3 (Int J Mol Cell Med 2025)
Int J Mol Cell Med 2025, 14(3): 808-827 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Mousavi S, Rashidi M, Azam K, Kouchak M, Salehi M, Mohammadzadeh G. Vitamin E incorporated in Carboxymethyl Cellulose-Gelatin Hydrogel Increases Wound Healing. Int J Mol Cell Med 2025; 14 (3) :808-827
URL: http://ijmcmed.org/article-1-2570-en.html
URL: http://ijmcmed.org/article-1-2570-en.html
Seyed-Reza Mousavi1 
, Mojtaba Rashidi2 , Khedri Azam3 , Maryam Kouchak4 , Majid Salehi5 , Ghorban Mohammadzadeh6
, Mojtaba Rashidi2 , Khedri Azam3 , Maryam Kouchak4 , Majid Salehi5 , Ghorban Mohammadzadeh6
1- Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Pharmaceutics, Faculty of Pharmacy, Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
5- Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
6- Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ,mohammadzadeh@ajums.ac.ir
2- Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3- Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Pharmaceutics, Faculty of Pharmacy, Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
5- Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
6- Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. ,
Abstract: (563 Views)
Improving wound care products to facilitate effective skin repair is very important. Hydrogels are promising polymer-based dressings that enhance wound healing. Vitamin E can improve skin injuries by increasing antioxidant capacity. This study aimed to construct an innovative hydrogel from carboxymethyl cellulose (CMC) and gelatin (Gel) containing vitamin E to enhance wound healing.
Five unique hydrogel formulations were constructed by combination of CMC-Gel and 25, 50, 100, and 200 µL/mL of Vitamin E. Structural characteristics of hydrogels were assessed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Biochemical properties of hydrogels, including swelling, weight loss, pH values, and blood compatibility, were evaluated by specific methods. The cytotoxicity effects of hydrogels on the NIH-3T3 fibroblasts were determined by MTT assay. The therapeutic potential of the hydrogels was investigated using a full-thickness wound model in Wistar rats. The constructed CMC-Gel-Vit E hydrogel had a porous structure characterized by interconnected voids measuring 73.15±9.61 µm, which is favorable for promoting cell migration. The cytotoxicity results showed no toxicity effects of hydrogels with and without VitE 100 µL/mL, also a survival rate of over 120% in cells after 72 h. In vivo data showed the CMC-Gel-Vit E hydrogel (91.36±8.23%) significantly increased the percentage of wound closure and re-epithelialization compared to the control group (68.31±13.59%).
The results highlight the considerable potential of the CMC-Gel-VitE hydrogel as a viable option for skin regeneration and wound healing. This hydrogel exhibits substantial promise for use in clinical and therapeutic interventions.
Five unique hydrogel formulations were constructed by combination of CMC-Gel and 25, 50, 100, and 200 µL/mL of Vitamin E. Structural characteristics of hydrogels were assessed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). Biochemical properties of hydrogels, including swelling, weight loss, pH values, and blood compatibility, were evaluated by specific methods. The cytotoxicity effects of hydrogels on the NIH-3T3 fibroblasts were determined by MTT assay. The therapeutic potential of the hydrogels was investigated using a full-thickness wound model in Wistar rats. The constructed CMC-Gel-Vit E hydrogel had a porous structure characterized by interconnected voids measuring 73.15±9.61 µm, which is favorable for promoting cell migration. The cytotoxicity results showed no toxicity effects of hydrogels with and without VitE 100 µL/mL, also a survival rate of over 120% in cells after 72 h. In vivo data showed the CMC-Gel-Vit E hydrogel (91.36±8.23%) significantly increased the percentage of wound closure and re-epithelialization compared to the control group (68.31±13.59%).
The results highlight the considerable potential of the CMC-Gel-VitE hydrogel as a viable option for skin regeneration and wound healing. This hydrogel exhibits substantial promise for use in clinical and therapeutic interventions.
Type of Study: Original Article |
Subject:
Histopathology
Received: 2025/03/28 | Accepted: 2025/06/3 | Published: 2025/10/1
Received: 2025/03/28 | Accepted: 2025/06/3 | Published: 2025/10/1
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
