Volume 0 - Int J Mol Cell Med (Inprees)                   Int J Mol Cell Med 2025, 0 - Int J Mol Cell Med (Inprees): 0-0 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jan Z, Norouzi Taheri H, Danesh A. Optimizing Production Conditions of a Caspian Sea Actinomycete Exhibiting Promising Antibacterial Activity Against Clinically-important Pathogens Using the Two-Factor Interaction/ Minimum Run Resolution IV Method. Int J Mol Cell Med 2025;
URL: http://ijmcmed.org/article-1-2445-en.html
1- Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran & Student Research Committee, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2- 3. Department of Biology Education, Farhangian University, Tehran, Iran
3- Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran & 4. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran , DaneshA@mums.ac.ir
Abstract:   (49 Views)

Natural products recovered from marine sediment have the potential for the treatment of various diseases. Streptomyces sp., strain MN38 which was previously isolated from the Caspian Sea of Iran was at first characterized based on its 16srRNA analysis and morphological properties. Two-factor Interaction/ Minimum Run Resolution IV method was employed to evaluate the influence of various potential factors on the strain's antibacterial activity using cost-effective substrates.
Twelve variables were considered effective for investigation, with an emphasis on assessing the antibacterial activity against Staphylococcus aureus and Enterococcus faecalis via the micro-dilution method. The findings indicate that a quadratic model and a second-order polynomial equation are statistically significant at the 95% confidence level due to a low P-value (< 0.0001) in this context.
As a result, A1BFe+C agar medium, incubated at 28°C for three days with a shaking speed of 200 rpm, using specific concentrations of starch (5.0 g/l), yeast extract (6.0 g/l), peptone (4.0 g/l), KBr (0.5 g/l), CaCO3 (0.2 g/l), sea salt (15 g/l), and Fe2(SO4)3 (0.003 g/l), along with an inoculum size of 3.0% v/v at a pH of 6, exhibits the enhanced antibacterial activity against Staphylococcus aureus and Enterococcus faecalis reached up to 69 and 166 (Bu/ml). It could be concluded that the MN38 of Caspian Sea sediments was a potent source of antimicrobial agent production and the production process was significantly optimized using mathematical methods.


 
Full-Text [PDF 884 kb]   (27 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2024/10/5 | Accepted: 2025/02/5 | Published: 2025/12/10

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | International Journal of Molecular and Cellular Medicine IJMCM

Designed & Developed by : Yektaweb