Dysregulation of LncRNAs ANRIL, MALAT1, and LINC00305 in Coronary Slow Flow Patients: Implications for Inflammation and Endothelial Dysfunction

Gheidari, Mohammad Esmail; Geramifard, Asal; Rafiei, Mahyar

doi:10.22088/IJMCM.BUMS.13.1.91

Volume 13, Issue 1 (Int J Mol Cell Med 2024) Int J Mol Cell Med 2024, 13(1): 91-104 | Back to browse issues page

‎ 10.22088/IJMCM.BUMS.13.1.91

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Gheidari M E, Geramifard A, Rafiei M. Dysregulation of LncRNAs ANRIL, MALAT1, and LINC00305 in Coronary Slow Flow Patients: Implications for Inflammation and Endothelial Dysfunction. Int J Mol Cell Med 2024; 13 (1) :91-104
URL: http://ijmcmed.org/article-1-2363-en.html

Dysregulation of LncRNAs ANRIL, MALAT1, and LINC00305 in Coronary Slow Flow Patients: Implications for Inflammation and Endothelial Dysfunction

Mohammad Esmail Gheidari¹

, Asal Geramifard²

, Mahyar Rafiei³

1- Department of Cardiology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2- Cardiovascular Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. & School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran , asalgeramifard@sbmu.ac.ir
3- Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

Abstract: (717 Views)

Coronary Slow Flow (CSF) is observed in individuals who experience delayed blood supply in the coronary arteries. Inflammation and endothelial dysfunction may play a role in the etiology and development of CSF. The current investigation aimed to compare the expression of specific long noncoding RNAs (lncRNAs) associated with endothelial dysfunction and inflammation in CSF patients. This case‒control study enrolled 72 CSF patients and 71 healthy individuals. Blood samples were collected, and serum marker levels were measured. The expression levels of lncRNAs ANRIL, MALAT1, and LINC00305 in peripheral blood mononuclear cells (PBMCs) were assessed using real-time Polymerase Chain Reaction (PCR). All statistical analyses were performed using SPSS 22, with the significance level set at P < 0.05. The study revealed that the relative expression of MALAT1 and LINC00305 was significantly lower in the CSF group (p < 0.01), whereas ANRIL was expressed at higher levels (p < 0.0001). The areas under the ROC curves (AUCs) for MALAT1, LINC00305, and ANRIL were 0.64, 0.66, and 0.75, respectively. Notably, the expression level of LINC00305 exhibited an inverse correlation with CSF incidence (OR: 0.83, p: 0.008) in contrast to that of ANRIL (OR: 1.43, p < 0.0001). Additionally, compared to those in the control group, the average BMI, WBC, RBC, Hb, LDH, LDL, FBS, and percentage of neutrophils in the CSF group were significantly greater (p< 0.05). lncRNA ANRIL is upregulated in CSF patients, whereas MALAT1 and LINC00305 are downregulated. Dysregulation of ANRIL, MALAT1, and LINC00305 may serve as diagnostic and predictive factors for CSF leakage.

Keywords: Coronary vessels, slow flow phenomenon, endothelium, inflammation, LncRNA

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Type of Study: Original Article | Subject: Genetics & Disease
Received: 2024/06/10 | Accepted: 2024/07/3 | Published: 2024/07/29