Volume 4, Issue 1 (Int J Mol Cell Med 2015)                   Int J Mol Cell Med 2015, 4(1): 60-66 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghatreh K, Farrokhi E, Niknam S. The Effect of Adiponectin on Osteonectin Gene Expression by Oxidized Low Density Lipoprotein-Treated Vascular Smooth Muscle Cells. Int J Mol Cell Med 2015; 4 (1) :60-66
URL: http://ijmcmed.org/article-1-233-en.html
1- Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2- Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. , e_farrokhi_k@yahoo.com
3- Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Abstract:   (9327 Views)
Osteonectin is a bone- associated protein involved in vascular calcification. Adiponectin may protect against cardiovascular disease but possible effects on vascular calcification have been poorly studied. The aim of this study was to investigate the modulatory effect of adiponectin on oxidized low density lipoprotein (oxLDL)- induced expression of osteonectin in human aorta vascular smooth muscle cells (HA/VSMCs). HA/VSMCs were cultured in F12K media and then treated with oxLDL (100 µg/mL) in the presence or absence of adoponectin (5 µg/mL) for 24 and 48 hours. mRNA expression and protein level of osteonectin were determined by quantitative real-time PCR and western blot analysis, respectively. After exposure to oxLDL, osteonectin expression increased 1.62 ± 0.23- and 6.62 ± 0.48-fold after 24 and 48 hours respectively compared to the control. Adiponectin increased oxLDL- induced osteonectin expression in a time-dependent manner after 24 and 48 hours (3.24 ± 0.39- and 24.93 ± 2.15-fold, respectively). Western blotting confirmed that osteonectin protein was upregulated by adiponectin.Our data suggest that OxLDL might cause the increase of osteonectin expression both at mRNA and protein level. This upregulation is intensified by adiponectin.
Full-Text [PDF 79 kb]   (3272 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2014/09/23 | Accepted: 2015/01/3 | Published: 2015/02/7

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2025 CC BY-NC 4.0 | International Journal of Molecular and Cellular Medicine IJMCM

Designed & Developed by : Yektaweb