Gene Expression of Glycolysis Enzymes in MCF-7 Breast Cancer Cells Exposed to Warburg Effect and Hypoxia

BAYAR, İrem; Ekren Aşıcı, Gamze Sevri; Bildik, Ayşegül; Kıral, Funda

doi:10.22088/IJMCM.BUMS.13.1.29

Volume 13, Issue 1 (Int J Mol Cell Med 2024) Int J Mol Cell Med 2024, 13(1): 29-45 | Back to browse issues page

‎ 10.22088/IJMCM.BUMS.13.1.29

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BAYAR İ, Ekren Aşıcı G S, Bildik A, Kıral F. Gene Expression of Glycolysis Enzymes in MCF-7 Breast Cancer Cells Exposed to Warburg Effect and Hypoxia. Int J Mol Cell Med 2024; 13 (1) :29-45
URL: http://ijmcmed.org/article-1-2223-en.html

Gene Expression of Glycolysis Enzymes in MCF-7 Breast Cancer Cells Exposed to Warburg Effect and Hypoxia

İrem BAYAR¹

, Gamze Sevri Ekren Aşıcı²

, Ayşegül Bildik²

, Funda Kıral²

1- Selcuk University Faculty of Veterinary, Department of Biochemistry Konya, Turkey. , irem.bayar@selcuk.edu.tr
2- Adnan Menderes University Faculty of Veterinary, Department of Biochemistry Aydın, Turkey.

Abstract: (1111 Views)

Hypoxia can cause significant changes in the glucose metabolism of cancer cells that prefer aerobic glycolysis for energy production instead of the conventional oxidative phosphorylation mechanism. In this study, breast cancer cells (MCF-7) were exposed to glucose (0-5.5-15-55 mM), during specific incubation periods (3, 6, 12, or 24 hours) under normoxic and hypoxic conditions. The expression levels of hypoxia-inducible factor-1α (HIF-1α), glucose transporter-1 (GLUT-1), and glycolytic enzymes at varying glucose concentrations in cells were investigated in the different oxygen environments. It was determined that glycolytic enzymes [Hexokinase 2 (HK2), Pyruvate Kinase M2 (PKM2), Glucose-6-phosphate dehydrogenase (G6PD), Lactate Dehydrogenase A (LDHA), Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH), and Phosphofructokinase M (PFKM)] increased at the transcriptional level, especially in the first hours. This increase indicates that major metabolic reprogramming in response to hypoxia probably occurs over a short period of time. The increase in G6PD gene expression under high glucose and hypoxia conditions suggests that the pentose phosphate pathway (PPP) is used by cancer cells to synthesize necessary precursors for the cell. The results of the study showed that there is a significant interaction between hypoxia and glycolytic metabolism in cancer cells. It is thought that metabolic pathways activated by hypoxia and related genes located in these pathways will contribute to the literature by offering the potential to be target molecules for therapeutic purposes.

Keywords: Glucose, glycolysis, gene expression, hypoxia, MCF-7, normoxia

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Type of Study: Original Article | Subject: Cancer
Received: 2023/09/20 | Accepted: 2024/06/30 | Published: 2024/07/30