Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8

Krishnan, Kannabiran; Mani, Abirami; Jasmine, Subashini

Volume 3, Issue 4 (Int J Mol Cell Med 2014) Int J Mol Cell Med 2014, 3(4): 246-254 | Back to browse issues page

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Krishnan K, Mani A, Jasmine S. Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8. Int J Mol Cell Med 2014; 3 (4) :246-254
URL: http://ijmcmed.org/article-1-218-en.html

Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8

Kannabiran Krishnan¹

, Abirami Mani²

, Subashini Jasmine²

1- School of Biosciences and Technology, VIT University, Tamilnadu, India. , kkb@vit.ac.in
2- School of Biosciences and Technology, VIT University, Tamilnadu, India.

Abstract: (11818 Views)

Marine Streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in pharmaceutical industry as effective drugs against life threatening diseases. The cytotoxic activity of the pure compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (DMEHE) from marine derived actinomycete Streptomyces sp. VITSJK8 was investigated against mouse embryonic fibroblast (NIH 3T3) and human keratinocyte (HaCaT) normal cell lines, human hepatocellular liver carcinoma (HepG 2) and human breast adenocarcinoma (MCF-7) cell lines by using MTT assay. The compound DMEHE exhibited IC 50 values of 42, 100, 250 and 500 µg/ ml against HepG2, MCF-7, HaCaT and NIH 3T3 cell lines, respectively. The effect of DMEHE on the growth of cancer cell lines was expressed as the % of viability. Cell viability was recorded as 67.7%, 78.14%, 82.23% and 96. 11% in HepG2, MCF-7, HaCaT and NIH 3T3 cells, respectively. The results of the study conclude that the bioactive compound isolated from the potential isolate Streptomyces sp. VITSJK8 exhibited cytotoxic activity against HepG2 and MCF- 7 cancer cell lines and low toxicity against normal HaCaT and NIH 3T3 cell lines.

Keywords: Streptomyces sp. VITSJK8, 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester, cytotoxicity, MTT assay

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Type of Study: Original Article | Subject: Other
Received: 2014/08/25 | Accepted: 2014/11/1 | Published: 2014/11/23