A highly pure sub-fraction of shallot extract with potent in vitro anti-angiogenic activity

Famil Samavati, Shima; Mohammadi-Motlagh, Hamid-Reza; Mostafaie, Ali

Volume 3, Issue 4 (Int J Mol Cell Med 2014) Int J Mol Cell Med 2014, 3(4): 237-245 | Back to browse issues page

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Famil Samavati S, Mohammadi-Motlagh H, Mostafaie A. A highly pure sub-fraction of shallot extract with potent in vitro anti-angiogenic activity. Int J Mol Cell Med 2014; 3 (4) :237-245
URL: http://ijmcmed.org/article-1-198-en.html

A highly pure sub-fraction of shallot extract with potent in vitro anti-angiogenic activity

Shima Famil Samavati¹

, Hamid-Reza Mohammadi-Motlagh²

, Ali Mostafaie³

1- Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran.
2- Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran. , mohammadimotlagh@gmail.com
3- Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract: (9390 Views)

Abstract Our previous studies showed that various extracts of Persian shallot (Allium hirtifolium) have anti-angiogenic effects. This study has been undertaken to isolate and identify the major effective anti-angiogeneic sub-fraction of shallot. After preparation of the 50% hydroalcoholic extract of shallot bulbs, the extract was successively fractionated into n-hexane, ethyl acetate, n-butanol and aqueous fractions. Anti-angiogenesis activity of fractions was examined by in vitro angiogenesis assay. The ethyl acetate fraction which had the most anti-angiogenesis activity was further fractionated to four sub-fractions by thin layer chromatography (TLC), silica gel column chromatography and then analyzed by High Performance TLC (HPTLC) with ethyl acetate-methanol-water as the solvent system. Our results showed that one of the four sub-fractions, as the major band in HPTLC, had the most anti-angiogenic activity. Purification and characterization of the major anti-angiogenic compound/compounds of shallot's extract may constitute one means by which diets rich in shallot confer protection against cancer and finally introduce new agents with pharmacological activities in shallot as a potential candidate in cancer therapy.

Keywords: Allium hirtifolium, Angiogenesis, Phytochemicals, Cancer, High performance thin layer chromatography

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Type of Study: Original Article | Subject: Cancer
Received: 2014/07/15 | Accepted: 2014/08/4 | Published: 2014/09/3