[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 11, Issue 1 (Int J Mol Cell Med (In Press) 2022) ::
Int J Mol Cell Med 2022, 11(1): 0-0 Back to browse issues page
ACE-2 Expression and Methylation Pattern in Bronchoalveolar Lavage Fluid and Bloods of Iranian ARDS Covid-19 Patients
Reza Najafipour1 , Davood Mohammadi2 , Mabood Momeni3 , Sahar Moghbelinejad 4
1- Genetics Research Center, University of Social Welfare and Rehabilitation Science, Tehran, Iran.
2- Department of Surgery, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
3- Khatam Pathobiology and Genetic Lab, Qazvin, Iran.
4- Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran. , smoghbelinejad@qums.ac.ir
Abstract:   (233 Views)

The aim of the present study was to investigate the expression and methylation pattern of the angiotensin I converting enzyme 2 (ACE-2) in acute respiratory distress syndrome (ARDS) covid-9 patients. A total of 25 patients with covid-19 ARDS and 20 controls were recruited. Expression of the ACE-2 gene was evaluated by quantitative real time PCR, and methylation of CpG dinucleotides, in the ACE-2 promoter, was quantified using bisulfite pyro-sequencing. Our results showed high expression of the ACE-2 gene in the blood samples of ADRS patients (1.93± 0.67) in comparison to controls (0.62±0.35) (p = 0.03). Correspondingly, in ARDS Bronchoalveolar lavage fluid (BALF) samples, there was a high expression of this gene (1.8±0.78) in comparison to controls (0.58±0.2) (p <0.05). Moreover, the methylation rate of the ACE-2 gene in blood samples of ARDS patients was 64.07 ±6.1 in comparison to controls (80.3 ±7.3) (p<0.0001).  In BALF samples, there was this pattern too (55.07 ±3.1 vs. 72.35±5.1) (p<0.0001). Finally, a significant correlation was found between expression and methylation in BALF (R= -0.54, P= 0.002) and blood (R= -0.321, P= 0.013) samples. These results indicated that aberrant methylation of the ACE-2 promoter might be associated with high expression of this gene and the occurrence of ARDS in covid-19 patients.

 

Keywords: Covid-19, ACE-2, expression, methylation
     
Type of Study: Original Article | Subject: Genetics & Disease
Received: 2022/02/14 | Accepted: 2022/08/22 | Published: 2022/06/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Najafipour R, Mohammadi D, Momeni M, Moghbelinejad S. ACE-2 Expression and Methylation Pattern in Bronchoalveolar Lavage Fluid and Bloods of Iranian ARDS Covid-19 Patients. Int J Mol Cell Med. 2022; 11 (1)
URL: http://ijmcmed.org/article-1-1867-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 11, Issue 1 (Int J Mol Cell Med (In Press) 2022) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.04 seconds with 30 queries by YEKTAWEB 4460