Histopathologic Responses of the Dental Pulp to Calcium-Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) in Diabetic and Non-Diabetic Rats

Madani, Zahra Sadat; Haddadi, Azam; Mesgarani, Abbas; Seyedmajidi, Maryam; Mostafazadeh, Amrollah; Bijani, Ali; Ashraphpour, Manouchehr

Volume 3, Issue 4 (Int J Mol Cell Med 2014) Int J Mol Cell Med 2014, 3(4): 263-271 | Back to browse issues page

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Madani Z S, Haddadi A, Mesgarani A, Seyedmajidi M, Mostafazadeh A, Bijani A et al . Histopathologic Responses of the Dental Pulp to Calcium-Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) in Diabetic and Non-Diabetic Rats. Int J Mol Cell Med 2014; 3 (4) :263-271
URL: http://ijmcmed.org/article-1-175-en.html

Histopathologic Responses of the Dental Pulp to Calcium-Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) in Diabetic and Non-Diabetic Rats

Zahra Sadat Madani¹

, Azam Haddadi²

, Abbas Mesgarani³

, Maryam Seyedmajidi¹

, Amrollah Mostafazadeh⁴

, Ali Bijani⁵

, Manouchehr Ashraphpour⁶

1- Dental Materials Research Center Dental School, Babol University of Medical Sciences, Babol, Iran.
2- Dental School, Mazandaran University of Medical Sciences, Sari, Iran. , ddadi_azam@yahoo.com
3- Dental School, Babol University of Medical Sciences Babol, Iran.
4- Cellular& Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
5- Non–Communicable Pediatric Diseases Research Center Babol University of Medical Sciences, Babol, Iran.
6- Department of pharmacology and Neurosciences, Babol University of Medical Sciences, Babol, Iran.

Abstract: (9082 Views)

Diabetes mellitus is a chronic disease which affects the healing ability of the pulp and periodontium. The aim of the present study was to assess the histopathologic response of dental pulp to pulp capping using MTA or CEM cement in diabetic rats. Thirty two Wistar male rats aged between 8 and 10 weeks (weight: 200-250g) were divided into two groups of diabetic (n=16) and healthy (n=16) animals and then subdivided into MTA and CEM subgroups. In each group, 10 MTA treated, 10 CEM treated and 12 intact (without any intervention) teeth were analyzed. Intact teeth were considered as a baseline inflammation control. Then, class I cavity was made in the maxillary first molars teeth with pinpoint pulpal exposure. Either MTA or CEM cement was then placed over exposed pulp as pulp capping agent and the cavities were restored using resin- modified glass ionomer cement. Both teeth of rats in subgroups remained intact without any intervention. After four weeks, the rats were sacrificed and the teeth were subjected to histological evaluation in terms of inflammation intensity, dentin bridge formation and dentin bridge continuity. The CEM cement treated diabetic rats exhibited a significant higher inflammatory response when compared to healthy control group (P=0.004) whereas, MTA treated diabetic rats did not exhibit a significant higher inflammatory response in comparison to healthy controls. There was no significant difference between MTA and CEM cement in the induction of dentin bridge formation in diabetic and healthy controls. This preliminary study suggests that MTA is a superior dental material than CEM cement for pulp therapy in subjects with diabetes.

Keywords: Diabetes, direct pulp capping, CEM cement, MTA

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Type of Study: Original Article | Subject: Histopathology
Received: 2014/06/11 | Accepted: 2014/09/15 | Published: 2014/10/1