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:: Volume 9, Issue 4 (Int J Mol Cell Med (In Press) 2020) ::
Int J Mol Cell Med 2020, 9(4): 0-0 Back to browse issues page
Aberrant Expression of Immune-related MicroRNAs in Pediatric Patients with Asthma
Hala Gouda Elnady1, Lobna Sayed Sherif1, Naglaa Mohamed Kholoussi2, Mona Ali Azzam3, Ahmed Rashad Foda1, Iman Helwa2, Rania Nabil Sabry1, Eman Eissa 4, Reham Faisal Fahmy1
1- Department of Child Health, Medical Research Division, National Research Center, Egypt.
2- Immunogenetics Department, Human Genetics and Genome Research Division, National Resrearch Center, Egypt.
3- Department of Pediatrics, Faculty of Medicine, Suez Canal University, Egypt; McMaster University, Hamilton, Canada.
4- Immunogenetics Department, Human Genetics and Genome Research Division, National Resrearch Center, Egypt. , emanessa82@yahoo.com
Abstract:   (101 Views)
MicroRNAs (miRNAs) have been implicated as regulatory molecules that could play a considerable role in the pathogenesis of different diseases including asthma. This work aims at exploring the role of miR-146a and miR-106b in the pathogenesis of asthma and their association with asthma severity, IgE, and inflammatory cytokines in asthmatic children. Thirty asthmatic children and twenty age-matched healthy children aged 4-17 years old were enrolled. Expression of plasma miR-146a and miR-106b was measured using quantitative real-time PCR. Plasma levels of interleukin-5 (IL-5) and interleukin-13 (IL-13) were assessed using ELISA. Lung functions were measured by Spirometry. MiR-146a and miR-106b were significantly over-expressed in asthmatic children compared to healthy children. A significant positive correlation between total IgE and both miR-146a and miR-106b was found while no significant correlation could be detected between these miRNAs and asthma severity in asthmatic children. Plasma levels of IL-5 and IL-13 were non-significantly higher in asthmatic children compared to healthy children, and there was no significant correlation between them and both miR-146a and miR-106b expressions in the asthmatic children.  The aberrant expression of immune-related miRNAs (miR-146a and miR-106b) and inflammatory cytokines (IL-5 and IL-13) among asthmatic children suggest their probable role in asthma pathogenesis.
Keywords: Asthma, microRNAs, cytokines, children, IL-5, IL-13
Full-Text [PDF 274 kb]   (17 Downloads)    
Type of Study: Original Article | Subject: Molecular & Cellular Immunology
Received: 2020/09/7 | Accepted: 2021/01/4 | Published: 2020/11/30
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Elnady H G, Sherif L S, Kholoussi N M, Azzam M A, Foda A R, Helwa I, et al . Aberrant Expression of Immune-related MicroRNAs in Pediatric Patients with Asthma. Int J Mol Cell Med. 2020; 9 (4)
URL: http://ijmcmed.org/article-1-1437-en.html


Volume 9, Issue 4 (Int J Mol Cell Med (In Press) 2020) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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