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:: Volume 9, Issue 3 (Int J Mol Cell Med 2020) ::
Int J Mol Cell Med 2020, 9(3): 207-214 Back to browse issues page
EL4-derived Exosomes Carry Functional TNF-related Apoptosis-inducing Ligand that are Able to Induce Apoptosis and Necrosis in the Target Cells
Sajjad Tavakkoli1 , Fattah Sotoodehnejadnematalahi1 , Anwar Fathollahi2 , Mojgan Bandehpour3 , Mostafa Haji Molla Hoseini2 , Farshid Yeganeh 4
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4- Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. , fyeganeh@gmail.com
Abstract:   (324 Views)
Exosomes released by tumor cells play critical roles in tumor progression, immune cell suppression, and cancer metastasis. The aim of the present study was to investigate whether the exosomes released by EL4 cells carry a functional TNF-related apoptosis-inducing ligand (TRAIL) molecule. Exosomes were harvested from the supernatants of EL4 cell culture, and the shape, size, and identity of EL4-derived exosomes were evaluated by utilizing scanning electron microscopy, dynamic light scattering, and dot-blot method. The expression of mRNA and TRAIL protein in EL4 cells and EL4-exosomes were investigated using real-time PCR method and dot-blot analysis. Moreover, the effects of EL4-derived exosomes on cell death in a TRAIL-sensitive cell line (4T1) were studied by using flow cytometry (annexin V/ propidium iodide (PI) staining) and fluorescent microscopy analyses (acridine orange/ethidium bromide staining). The results showed that EL4 cells continuously and without the need for stimulation, produce exosomes that carry TRAIL protein. In addition, EL4-derived exosomes were capable to induce apoptosis as well as necrosis in 4T1 cells. It was ultimately revealed that EL4 cells express TRAIL protein and release exosomes containing functional TRAIL. Moreover, the released exosomes were able to induce apoptosis and necrosis in a TRAIL-sensitive cell line. Further studies are needed to reveal the potential roles of tumor-derived exosomes in the pathogenesis of cancers.
Keywords: Apoptosis, exosomes, necrosis, TNF-related apoptosis-inducing ligand, tumor
Full-Text [PDF 478 kb]   (101 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2020/04/17 | Accepted: 2020/10/31 | Published: 2020/08/20
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Tavakkoli S, Sotoodehnejadnematalahi F, Fathollahi A, Bandehpour M, Haji Molla Hoseini M, Yeganeh F. EL4-derived Exosomes Carry Functional TNF-related Apoptosis-inducing Ligand that are Able to Induce Apoptosis and Necrosis in the Target Cells. Int J Mol Cell Med. 2020; 9 (3) :207-214
URL: http://ijmcmed.org/article-1-1304-en.html


Volume 9, Issue 3 (Int J Mol Cell Med 2020) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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