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:: Volume 9, Issue 1 (Int J Mol Cell Med 2020) ::
Int J Mol Cell Med 2020, 9(1): 73-82 Back to browse issues page
Saponins from Tribulus terrestris L. Extract Down-regulate Expression of ICAM-1, VCAM-1 and E-selectin in Human Endothelial Cell Lines
Zahra Fereydouni1 , Elahe Amirinezhad Fard1 , Kamran Mansouri1 , Hamid-Reza Mohammadi Motlagh1 , Ali Mostafaie 2
1- Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2- Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. , amostafaie@kums.ac.ir
Abstract:   (505 Views)
Atherosclerosis is an inflammatory disease in which intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin (SELE) are consistently expressed in the vascular endothelium. Several evidence support the crucial role of adhesion molecules in the development of atherosclerosis and plaque instability. Due to the anti-inflammatory activity of Tribulus terrestris (TT), the present study investigated the effect of aqueous extract and saponin fraction of TT on the expression of ICAM-1, VCAM-1, and SELE genes in endothelial cells during normal and lipopolysaccharide (LPS) induced conditions. Human umbilical vein endothelial cells (HUVEC) and human bone marrow endothelial cells (HBMEC) were cultured, stimulated by LPS, and treated with aqueous extract and saponin fraction of TT. Finally, the expression of ICAM-1, VCAM-1, and SELE genes were measured using quantitative real-time polymerase chain reaction. LPS-induced HUVECs and HBMECs significantly increased the expression of ICAM-1, VCAM-1, and SELE in comparison with control groups (P<0.001). Treatment of LPS-induced HUVECs and HBMECs by aqueous extract and saponin fraction of TT decreased the expression of all three mentioned genes significantly (P<0.001) in comparison with LPS-induced cells. Taken together, our data suggest that TT has an anti-inflammatory effect. In vivo study about anti-inflammatory effect of this herb may provide new insights into the development of a herbal drug for the prevention/therapy of atherosclerosis.
Keywords: Atherosclerosis, Tribulus terrestris L., gene expression, adhesion molecules, human endothelial cells, saponin
Full-Text [PDF 608 kb]   (122 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2020/02/8 | Accepted: 2020/05/10 | Published: 2020/04/29
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Fereydouni Z, Amirinezhad Fard E, Mansouri K, Mohammadi Motlagh H, Mostafaie A. Saponins from Tribulus terrestris L. Extract Down-regulate Expression of ICAM-1, VCAM-1 and E-selectin in Human Endothelial Cell Lines. Int J Mol Cell Med. 2020; 9 (1) :73-82
URL: http://ijmcmed.org/article-1-1255-en.html


Volume 9, Issue 1 (Int J Mol Cell Med 2020) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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