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:: Volume 9, Issue 1 (Int J Mol Cell Med 2020) ::
Int J Mol Cell Med 2020, 9(1): 62-72 Back to browse issues page
Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment
Ameneh Mehri-Ghahfarrokhi1, Mahammad-Saeid Jami2, Morteza Hashemzadeh Chaleshtori 3
1- Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. & Department of Neurology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA, USA.
3- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. , mhashemzadehskums@gmail.com
Abstract:   (980 Views)
Previous studies have shown that miR-124 plays an important role in the development of auditory neurons, which are degenerated in the sensorineural hearing loss. However, whether the combined use of miR-124 and growth factors can increase the expression of neural related markers in human dental pulp stem cells is unknown so far. In this study, human dental pulp stem cells were transfected with miR-124 following treatment with brain-derived neurotrophic factor or epidermal growth factor/basic fibroblast growth factor. The expression of some neural related markers (nestin, SOX2, β-tubulin III, MAP2, and peripherin) was analyzed in two groups by qRT-PCR or immunofluorescence. Cellular treatment resulted in morphological changes including neurosphere-like colonies formation. Nestin and SOX2 were up-regulated, and MAP2 and peripherin were down-regulated in dental pulp stem cells transfected by miR-124 following treatment with brain-derived neurotrophic factor. Replacement of brain-derived neurotrophic factor with epidermal growth factor/ basic fibroblast growth factor resulted in the up-regulation of nestin, MAP2, peripherin, and β-tubulin III and down-regulation of SOX2. The expression of SOX2 and nestin was also confirmed by immunofluorescence. The combination of miR-124 and growth factors would provide a promising starting point for upregulating the neural progenitor markers in human dental pulp stem cells.
Keywords: Spiral ganglion neuron, miR-124, dental pulp stem cells, brain-derived neurotrophic factor, auditory neuropathy.
Full-Text [PDF 789 kb]   (163 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2019/12/20 | Accepted: 2020/05/7 | Published: 2020/04/29
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Mehri-Ghahfarrokhi A, Jami M, Hashemzadeh Chaleshtori M. Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment. Int J Mol Cell Med. 2020; 9 (1) :62-72
URL: http://ijmcmed.org/article-1-1218-en.html


Volume 9, Issue 1 (Int J Mol Cell Med 2020) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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