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:: Volume 9, Issue 2 (Int J Mol Cell Med 2020) ::
Int J Mol Cell Med 2020, 9(2): 146-153 Back to browse issues page
HSV-TK Expressing Mesenchymal Stem Cells Exert Inhibitory Effect on Cervical Cancer Model
Azra Kenarkoohi1, Taravat Bamdad 2, Masoud Soleimani3, Hoorieh Soleimanjahi4, Ali Fallah5, Shahab Falahi6
1- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran; Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
2- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. , bamdad_t@modares.ac.ir
3- Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
4- Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
5- RNAx Ltd., London, UK.
6- Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Abstract:   (280 Views)
A growing area of research is focused on cancer therapy, and new therapeutic approaches are welcomed. Mesenchymal stem cell (MSC)-based gene therapy is a promising strategy in oncology. Intrinsic tropism and migration to tumor microenvironment with off lights are attractive features of this type of cell carrier. In this way, suicide genes have also found a good platform for better performance and have shown a stronger anti-tumor mechanism by riding on mesenchymal cells. In this study, we investigated the anti-tumor activity of intratumoral injected MSCs transduced with a lentivector expressing the HSV/TK in a mouse cervical cancer model. Following the injection of MSCs transduced with lentivector carrying TK, MSCs alone or PBS into the mice tumor, ganciclovir was administered intraperitoneally during 14 days, and tumor size, survival time, natural killer (NK) cells and cytotoxic T lymphocyte (CTL) activities were assessed. We demonstrated that combination of suicide therapy and cell therapy leading m,to successful tumor inhibition. Significant reduction in tumor size was detected in test group in comparison with controls. Also, potent antitumor NK and CTL activity was seen in treatment group in comparison with controls. Our data demonstrated that the mesenchymal cells expressing TK had inhibitory effect on cervical cancer model.
Keywords: Cervical cancer, mesenchymal stem cell, suicide therapy, lentivector, thymidine kinase, cell therapy
Full-Text [PDF 443 kb]   (78 Downloads)    
Type of Study: Original Article | Subject: Cancer
Received: 2019/09/4 | Accepted: 2020/06/23 | Published: 2020/07/5
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Kenarkoohi A, Bamdad T, Soleimani M, Soleimanjahi H, Fallah A, Falahi S. HSV-TK Expressing Mesenchymal Stem Cells Exert Inhibitory Effect on Cervical Cancer Model. Int J Mol Cell Med. 2020; 9 (2) :146-153
URL: http://ijmcmed.org/article-1-1154-en.html


Volume 9, Issue 2 (Int J Mol Cell Med 2020) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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