دوره 11، شماره 4 - ( 4-1401 )                   | برگشت به فهرست نسخه ها


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Alipour A H, Hashemi S M A, Moattari A, Farhadi A, Sarvari J. Epstein-Barr Virus Nuclear Antigen 1 Increases the Expression of Oncogenes and Cellular Genes in the HeLa Cell Line. Int J Mol Cell Med 2022; 11 (4) :346-356
URL: http://ijmcmed.org/article-1-2154-fa.html
Epstein-Barr Virus Nuclear Antigen 1 Increases the Expression of Oncogenes and Cellular Genes in the HeLa Cell Line. مجله بین المللی سلولی و مولکولی. 1401; 11 (4) :346-356

URL: http://ijmcmed.org/article-1-2154-fa.html


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Epstein-Barr virus (EBV) represents one of the most important viral carcinogens. EBV nuclear antigen-1 (EBNA1) can induce the expression of different cellular and viral genes. In this study, we evaluated the EBNA1 effects on the expression patterns of human papillomavirus type 18 (HPV-18) E6 and E7 oncogenes and three cellular genes, including BIRC5, c-MYC, and STMN1, in a cervical adenocarcinoma cell line. HeLa cells were divided into three groups: one transfected with a plasmid containing the EBNA1 gene, one transfected with a control plasmid, and one without transfection. In all three groups, the expression levels of E6, E7, BIRC5, c-MYC, and STMN1 genes were checked using real-time PCR. Pathological staining was used to examine changes in cell morphology. Real-time PCR results showed that the expression level of HPV-18 E6 (P=0.02) and E7 (P=0.02) oncogenes significantly increased in HeLa cells transfected with the EBNA1 plasmid compared to cells transfected with control plasmid. Also, the presence of EBNA1 induced the expression of BIRC5 and c-MYC, which increased tenfold (P=0.03) and threefold (P=0.02), respectively. Regarding the STMN1 cellular gene, although the expression level in HeLa cells transfected with EBNA1 plasmid showed a twofold increase, this change was insignificant (P=0.11). Also, EBNA1 expression caused the creation of large HeLa cells with abundant cytoplasm and numerous nuclei. The EBV-EBNA1 could increase the expression levels of HPV-18 E6 and E7 viral oncogenes as well as c-MYC and BIRC5 cellular genes in the HeLa cell line. These findings indicate that the simultaneous infection of cervical cells with HPV-18 and EBV might accelerate the progression of cervical cancer.
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نوع مطالعه: Original Article | موضوع مقاله: Cancer
دریافت: 1402/2/18 | پذیرش: 1402/5/2 | انتشار: 1402/5/4

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