Showing 3 results for Elmi
Hassan Hoda, Fatemeh Elmi, Maryam Mytra Elmi,
Volume 2, Issue 1 (Int J Mol Cell Med 2013)
Abstract
ABSTRACT
Background
Antibiotic resistance makes antimicrobial peptides (AMPs) agents an alternative for treatment of pathogenic diseases. They are isolated from various animals invertebrates, vertebrates and plants. The present study shows the electrophoretic pattern of protein and peptides from Vicia Faba seed and reports our first attempt to study the antibacterial activity of Vicia faba seed extract.
Methods
100g grounded seed was extracted in extraction buffer(10 mM Na2HPO4, 15 mM NaH2PO4, 100mM KCl, 2 mM EDTA, 2mM Thiurea 1.5% PVPP). Following a 70% ammonium sulfate precipitation was carried out. The pellet was dialyses and resuspended in 50mM ammonium acetate, then used for gel filtration chromatography (Sephadex G75). The crude extract electrophoresis was carried out on 12% SDS- PAGE gel. Antibacterial activity on E.Coli and B. Subtilis from hospital infection was tested and evaluated by measuring the inhibition zone diameter observation.
Results
The SDS- PAGE gel electrophoresis shows the crude extract contains many proteins with different spectrum molecular weight of proteins and peptides. The inhibition zone doesn’t observe in antibacterial properties tests. So, our experiments don’t show any antibacterial activity on E.Coli and B. Subtilis from hospital infection.
Conclusion
In our study, bacteria from hospital infection resists to Vicia faba seed extract. Some other AMPs haven’t also shown any antimicrobial properties on clinical trail. To show the antibacterial activity of the crude Vicia faba seed extract, it should be tested on some standard bacteria.
Hadis Musavi, Fariba Mohamadi Tahroodi, Farzaneh Fesahat, Zinatossadat Bouzari, Seddigheh Esmailzadeh, Fatemeh Elmi, Shahla Yazdani, Zoleika Moazezi,
Volume 8, Issue 3 (Int J Mol Cell Med 2019)
Abstract
Gestational diabetes mellitus (GDM) is defined as one of the three main types of diabetes mellitus (DM). It is established that GDM is associated with exceeding nutrient losses owing to glycosuria. Magnesium (Mg), as one of the essential micronutrients for fetus development, acts as the main cofactor in most enzymatic processes. The aim of this study was to measure serum and cellular levels of Mg, albumin, creatinine, and total protein to further clarify the relationship between these components and DM in pregnant women. Blood samples were obtained from 387 pregnant women. The participants were classified into four groups based on their type of diabetes, namely GDM (n=96), DM (n=44), at high-risk of DM (n=122), and healthy controls (n=125). All participants' fasting blood sugar (FBS), creatinine, albumin, Mg, and total protein in the serum levels and red blood cell Mg (RBC-Mg) were measured during 24-28 weeks of gestation. Groups were compared for a possible association between DM and abortion, gravidity, and parity. The serum levels of creatinine, FBS, albumin, Mg, and RBC-Mg were statistically different among the four groups (P = 0.001). Significant lower levels of RBC-Mg was observed in all studied groups in comparison with controls. Given a positive correlation between DM and abortion, it seems that decreased levels of RBC-Mg and serum albumin can increase the risk of abortion in pregnant women. Our data demonstrated significant alterations in albumin, Mg, and creatinine concentrations in women with DM or those at high risk of DM during their gestational age. It seems that the measurement of these biochemical parameters might be helpful for preventing the complications, and improving pregnancies outcomes complicated with DM.
Khadijeh Elmizadeh, Ali Homaei, Ensiyeh Bahadoran, Farzaneh Abbasi, Sahar Moghbelinejad,
Volume 12, Issue 4 (Int J Mol Cell Med 2023)
Abstract
The significant functional role of circular RNAs (circRNAs) in the progression of malignant tumors, including ovarian cancer, has been shown in various studies. In this study, we aimed to investigate the abnormal expression of hsa_circ_0008285 and its role in ovarian cancer pathogenesis. Quantitative real time polymerase chain reaction (qRT-PCR) and Western blot methods were used to detect the expression of hsa_circ_0008285 and some target genes in ovarian cancer tissues and related cell lines. To determine the functional roles of hsa_circ_0008285 in ovarian cancer, cell proliferation, apoptosis, and cell invasion assays were performed. Bioinformatics (Target scan, circ intractome) and luciferase reporter analyses were used to predict target genes. Results: In the present study, we first found that hsa_circ_0008285 was up regulated in ovarian cancer tissues and related cell lines. Bioinformatics, experimental data, and luciferase reporter analysis data showed miR-211-5p is a direct target of hsa_circ_0008285, while SIRT-1 is a direct target of miR-211-5p. Overexpression of hsa_circ_0008285 in cancer cells increased the expression of SIRT-1 and progression of cancer cells. Based on these results, inhibition of hsa_circ_0008285 expression could cause upregulation of miR-211-5p and down regulation of SIRT-1 and inhibited the proliferation and invasion of ovarian cancer cells. Conclusion: The results of the present study revealed that hsa_circ_0008285 suppressed ovarian cancer progression by regulating miR-211-5p expression to inhibit SIRT-1 expression.
