:: Volume 4, Issue 4 (Int J Mol Cell Med 2015) ::
Int J Mol Cell Med 2015, 4(4): 218-226 Back to browse issues page
An HIV-1 Mini Vaccine Induced Long-lived Cellular and Humoral Immune Responses
Mehdi Mahdavi 1, Massoumeh Ebtekar2, Zuhair Mohammad Hassan3, Sobhan Faezi4, Hamidreza Khorram Khorshid5, Morteza Taghizadeh6, Keyhan Azadmanesh7
1- Department of Immunology, Pasteur Institute of Iran, Tehran, Iran. , mahdavivac@gmail.com
2- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
3- Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4- Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
5- Genetics Research Centre, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
6- Department of Medical Virology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
7- Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
Abstract:   (4951 Views)

Memory formation is the most important aspect of a vaccine which can guarantee long-lasting immunity and protection. The main aim of the present study was to evaluate the memory immune responses after immunization with a mini vaccine. Mice were immunized with human immunodeficiency virus-1 P24-Nef fusion peptide and then cellular and humoral immune responses were evaluated. In order to determine long-lived memory, immune responses were monitored for 20 weeks after final immunization. The results showed that the candidate vaccine induced proliferation and cytotoxic T lymphocyte responses and shifted cytokine patterns to T helper-1 profile. Evaluation of humoral immune responses also showed an increase in total peptide specific-IgG titer and a shift to IgG2a humoral response. Monitoring of immune responses at weeks 4, 12 and 20 after last immunization showed that immunologic parameters have been sustained for 20 weeks. Our findings support the notion that long-lived memory responses were achieved using a mini vaccine immunization.

Keywords: BALB/c mice, HIV-1, long-lived immune responses, P24-Nef fusion peptide
Full-Text [PDF 993 kb]   (2091 Downloads)    
Type of Study: Original Article | Subject: Molecular & Cellular Immunology
Received: 2015/09/28 | Accepted: 2015/12/9 | Published: 2015/12/26


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Volume 4, Issue 4 (Int J Mol Cell Med 2015) Back to browse issues page